PARONI RITA CLARA

Biochemistry, role and nutritional status of Vitamin K in humans. Set up of specific analytical methods by HPLC for both vitamin K1 (phylloquinone) and vitamin K2 to investigate about the nutritional intake and the possible in different subjects (2008 - 2011)

Cholesterol metabolism. Studies on the activity of the key enzymes involved. (1985 - 1988)

Definitive and reference methods in clinical chemistry and microbiology. Study on creatinine, glucose, glycated hemoglobin, agar diffusion tests. (1990 - 2004)

Development of "omics" techniques for the study of complex lipids (ceramides, sphingomyelins, etc) in tissues and plasma. Applications to animal models of related diseases and treatment schemes for the modulation of the involved metabolic pathways. (2014 - )

Development of "omics" techniques for the study of the metabolism of melatonin, tryptophan and related indole compounds. Applications to complex matrices of vegetable and human origin. a) Study of the metabolic activities of specific high-melatonin-producing microorganisms to transform vegetable matrices into semi-finished products for functional foods intended for subjects affected by allergies and / or food intolerances. b) Studies of bioavailability and efficacy of melatonin in humans, administered according to different routes and different pharmaceutical formulations. c) Study of the whole indole pattern in the plasma of subjects subjected to deregulation of dark-light cycles. d) Study of antitumor efficacy in animals using innovative routes of administration. (2017 - )

Development of a method by LC-MS / MS for the assay of Aspirin and of the metabolite salicylic acid in plasma. Applications: a) Study of the pharmacokinetics and pharmacodynamics (production of tromboxane A2) of gastro resistant aspirin (slow-release) and plain aspirin in control subjects and in subjects affected by essential thrombocythemia, in order to identify the causes of aspirin resistance observed in these patients. b) Study of the pharmacokinetics and pharmacodynamics of different aspirin formulations (gastroprotecedt and) in pregnant women at risk of pre-eclampsia: evaluation of the prevalence of aspirin resistance and identification of the best formulation c) Evaluation of salicylic acid circulating levels, metabolic-inflammatory profile and intake of fruits and vegetables in children with essential obesity: changes after 1 year of nutritional-behavioral intervention. (2015 - )

Evaluation of the diagnostic effectiveness of new procedures for the early identification of oral cancers in patients with established risk factors (2017 - )

Evaluation of the pharmacokinetics and metabolic activity of Myriocin, natural inhibitor of the synthesis of ceramides, in different animal models and with different administration patterns. Development of analytical methods in LC-MS / MS for the study of the molecule in plasma and animal tissues. (2014 - )

Evaluation of the pharmacokinetics and metabolism of Paclitaxel in orthotopic mouse model of neuroblastoma after intraperitoneal or subcutaneous administration in lipid matrix (Lipogems-PTX). Development of a method by LC-MS / MS for the dosage of paclitaxel and of the main metabolites extracted from plasma and adipose cells (6-hydroxypaclitaxel and 3-hydroxypaclitaxel). (2018 - )

Identification of DNA mutations associated to different pathologies by using chromatographic methods (DHPLC). Set up of analytical methods by LC-MS to study the incorporation of the modified nucleotide isopenthenyladenosine into tRNA. (2004 - 2006)

Plasmatic, urinary and intra-erythrocyte metabolism of the antiretroviral Ribavirin and set up of specific analytical methods by HPLC. Evaluation of the correlation toxicity-activity in HCV or HCV-HIV patients. (1989 - )

Proteins and peptide purification and characterization. Studies on glycated albumin, collagenase, carbohydrate deficient transferrin (CDT), RANTES, insulin, pro-insulin, growth hormon. MHC, SDF-1alfa (1997 - 2002)

Role of asymmetric and symmetric dimethyl arginine (ADMA and SDMA) in critical ill patients with severe or sepsis septic shock. Specific studies on metabolism of hydroxyproline, 3-methyl histidine, homocisteine, cysteine. (1988 - )

Studies on oxidative stress and ischemia-reperfusion injury. Free radical damage in various pathological states in humans and in the preservation of food quality. In particular: a)Studies on the effect of perioperative hyperoxia on markers of oxidative stress in a population of patients undergoing major abdominal surgery. b)Studies on brain adaptation to hypoxia or hyperoxia in mouse models. c) Studies on ischemia-reperfusion injury in the kidney of rats and in the human heart. d) in vitro studies on the modulation of xanthine oxidase activity and on the influence of bioactive molecules on lipid peroxidation. Development of specific analytical methods. (1988 - )

Study of alterations of intestinal permeability in relation to diabetic pathology in humans. Development and optimization of methodologies based on capillary electrophoresis and liquid chromatography-mass spectrometry (LC-MS) for the study of intestinal permeability to lactulose and mannitol (2000 - 2006)