Data di Pubblicazione:
2023
Citazione:
Flow bioprocessing of citrus glycosides for high-value aglycone preparation / A. Colacicco, G. Catinella, C. Pinna, A. Pellis, S. Farris, L. Tamborini, S. Dallavalle, F. Molinari, M. Contente, A. Pinto. - In: CATALYSIS SCIENCE & TECHNOLOGY. - ISSN 2044-4753. - 13:15(2023 Aug 07), pp. 4348-4352. [10.1039/d3cy00603d]
Abstract:
An efficient one-pot, 2-step flow bioprocess for the hydrolysis of hesperidin (HES) and rutin (RT), citrus rutinosyl flavonoids, has been developed for the obtainment of the corresponding aglycones. A commercially available & alpha;-rhamnosidase (RN) and an extremophilic home-made & beta;-glycosidase (HOR) have been co-immobilized on glyoxyl-agarose beads to prepare a high-performing multi-active biocatalyst (imm-RN-HOR). After the optimization of the reaction conditions in batch mode, a "flow switch" was applied, increasing the productivity (>99% m.c., 5 min), cost-efficiency and sustainability of the overall process. Due to the recovery and reuse of all the materials involved in the flow biotransformation, this strategy is effectively a zero-waste process.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
A. Colacicco, G. Catinella, C. Pinna, A. Pellis, S. Farris, L. Tamborini, S. Dallavalle, F. Molinari, M. Contente, A. Pinto
Link alla scheda completa:
Link al Full Text: