Persona
CAPPELLETTI GRAZIELLA
PROFESSORE ORDINARIO
Settori (8)
Parole chiave (20)
ALFA-SINUCLEINA
CITOSCHELETRO
CITOSCHELETRO NEURONALE
CYTOSKELETON
DIFFERENZIAMENTO NEURONALE
DINAMICA DEI MICROTUBULI
IMAGING.
MALATTIA DI PARKINSON
MECCANISMI MOLECOLARI DI NEURODEGENERAZIONE
MICROTUBULE DYNAMICS
MICROTUBULI
MOLECULAR MECHANISMS OF NEURODEGENERATION
MORBO DI PARKINSON
NEURODEGENERAZIONE
NEURONAL DIFFERENTIATION
NEURONI DOPAMINERGICI
NEUROPROTECTIVE AGENTS
NEUROPROTEZIONE
PARKINSON'S DISEASE
TRASPORTO ASSONALE
No Results Found
Descrizione linee ricerca
Ruolo della disfunzione microtubulare nella Malattia di Parkinson (2015 - )
No Results Found
Ricerca finanziata (7)
Controllo epigenetico e teratogenesi: studio del ruolo di acetilazione e metilazione istonica nell'induzione di malformazioni assili da parte di acido valproico.
PUR20062008 - PUR 2006-2008
Progetto
Partecipante
2008
Dalla esposizione in utero alle malformazioni congenite: studio dei meccanismi e delle vie patogenetiche alla base delle malformazioni indotte da acido valproico nel topo.
PUR90 - PUR 90%
Progetto
Partecipante
2009
Prodromal DEtErminants for PhENoconversion of idiopathic RBD to alpha-synucleinopathies (PD, DLB and MSA) [DEEPEN-iRBD]
FON_NAZ - Bandi Altre Fondazioni
Progetto
Responsabile scientifico
2023
36 mesi
Studio dell¿espressione genica e proteica nella genesi di malformazioni craniofacciali ed assili indotte da fungicidi azolici in embrioni di roditore ed anfibio
PUR20062008 - PUR 2006-2008
Progetto
Partecipante
2006
Studio dell¿inibizione delle deacetilasi istoniche embrionali come meccanismo alla base delle malformazioni indotte nel topo da acido salicilico.
PUR20062008 - PUR 2006-2008
Progetto
Partecipante
2007
The role of Clusterin in Parkinson’s disease pathology: from the animal model generation to the target validation
INTLI - Finanziamenti internazionali
Progetto
Responsabile scientifico
2022
24 mesi
Tuning Tubulin Dynamics and Interactions to Face Neurotoxicity: a Multidisciplinary Approach for Training and Research
Horizon 2020_Education Joint Doctorate
Progetto
Responsabile scientifico
2019
48 mesi
No Results Found
Pubblicazioni (106)
Brevetti
Collegi di dottorato (17)
Università degli Studi di MILANO -
BIOLOGIA MOLECOLARE E CELLULARE-2023
(ciclo: 39 - Anno: 2023
2023
)
Università degli Studi di MILANO -
BIOLOGIA MOLECOLARE E CELLULARE-2022
(ciclo: 38 - Anno: 2022
2022
)
Università degli Studi di MILANO -
BIOLOGIA MOLECOLARE E CELLULARE-2021
(ciclo: 37 - Anno: 2021
2021
)
Università degli Studi di MILANO -
BIOLOGIA MOLECOLARE E CELLULARE-2020
(ciclo: 36 - Anno: 2020
2020
)
Università degli Studi di MILANO -
BIOLOGIA MOLECOLARE E CELLULARE-2019
(ciclo: 35 - Anno: 2019
2019
)
Università degli Studi di MILANO -
BIOLOGIA MOLECOLARE E CELLULARE-2018
(ciclo: 34 - Anno: 2018
2018
)
Università degli Studi di MILANO -
BIOLOGIA MOLECOLARE E CELLULARE-2017
(ciclo: 33 - Anno: 2017
2017
)
Università degli Studi di MILANO -
BIOLOGIA MOLECOLARE E CELLULARE-2016
(ciclo: 32 - Anno: 2016
2016
)
Università degli Studi di MILANO -
BIOLOGIA MOLECOLARE E CELLULARE-2015
(ciclo: 31 - Anno: 2015
2015
)
Università degli Studi di MILANO -
BIOLOGIA MOLECOLARE E CELLULARE-2014
(ciclo: 30 - Anno: 2014
2014
)
Università degli Studi di MILANO -
BIOLOGIA MOLECOLARE E CELLULARE-2013
(ciclo: 29 - Anno: 2013
2013
)
Università degli Studi di MILANO -
SCUOLA DI DOTTORATO IN SCIENZE BIOLOGICHE E MOLECOLARI-2012
(ciclo: 28 - Anno: 2012
2012
)
Università degli Studi di MILANO -
SCIENZE BIOLOGICHE E MOLECOLARI-2011
(ciclo: 27 - Anno: 2011
2011
)
Università degli Studi di MILANO -
SCIENZE BIOLOGICHE E MOLECOLARI-2010
(ciclo: 26 - Anno: 2010
2010
)
Università degli Studi di MILANO -
SCIENZE BIOLOGICHE E MOLECOLARI-2009
(ciclo: 25 - Anno: 2009
2009
)
Università degli Studi di MILANO -
BIOLOGIA CELLULARE E MOLECOLARE-2008
(ciclo: 24 - Anno: 2008
2008
)
Università degli Studi di MILANO -
BIOLOGIA CELLULARE E MOLECOLARE-2007
(ciclo: 23 - Anno: 2007
2007
)
No Results Found
Tutoraggio (11)
tutorship -
Dottorandi
- NOVELLO CLAUDIA
(01/10/2022 - )
20221001
tutorship -
Dottorandi
- ATTANASIO SIMONE
(01/10/2020 - )
20201001
tutorship -
Dottorandi
- BASELLINI MILO JARNO
(01/10/2020 - )
20201001
tutorship -
Dottorandi
- COMINCINI ALESSANDRO
(01/10/2019 - 30/03/2023)
20191001
tutorship -
Dottorandi
- KOTHUIS JOSINE MARIE
(01/10/2019 - 22/09/2023)
20191001
tutorship -
Dottorandi
- PIZZI SARA
(01/10/2019 - 30/03/2023)
20191001
tutorship -
Assegnisti
- MARANGON JACOPO
(01/08/2016 - 14/12/2016)
20160801
tutorship -
Dottorandi
- CANTELE FRANCESCA ORTENSIA
(01/11/2014 - 22/06/2018)
20141101
tutorship -
Assegnisti
- MARANGON JACOPO
(01/08/2014 - 31/07/2016)
20140801
tutorship -
Dottorandi
- DE GREGORIO CARMELITA
(01/11/2012 - 29/11/2016)
20121101
tutorship -
Dottorandi
- CASAGRANDE FRANCESCA
(01/11/2011 - 30/11/2015)
20111101
No Results Found
Description
Graziella Cappelletti is Associate professor of Human Anatomy, tenured position, Faculty of Science and Technology, University of Milano.
I have proven expertise in the field of cell biology of the cytoskeleton and its pivotal role in health and disease. In the first part of my career, I moved from purified proteins to cell cultures and mice models and built a strong experience in studying the microtubule system, its organization and dynamics, in gene- and toxin-based models of Parkinson's disease. I investigated the molecular mechanisms underlying neurodegeneration through multiple approaches ranging from in vitro assays to biochemistry and microscopy analyses in cultured neurons and brain sections by means of over-expression, silencing or incubation with recombinant proteins. In addition, live cell imaging and video-microscopy is used to look at microtubule dynamics and microtubule-dependant processes crucial to maintain neuronal functions, including vesicles recycling and axonal transport. In last years, I became extremely interested in the study of microtubule system and its regulation in human tissues as well as its role in neurodegeneration. My team has been working on a range of human cellular models such as skin fibroblasts, mesenchymal stem cells and induced-Pluripotent Stem Cells, and in human tissues including brain and skin biopsy. We contributed to a novel scenario in which microtubule dysfunction plays a crucial role in the pathogenesis of Parkinson’s disease. The numerous invitations for talks I received remarks the relevance of my work. I have also organized the Symposium S61 “Insights into early cellular dysfunctions leading to synucleinopathies” (7th Mediterranean Neuroscience Society, 2019). Finally, I received funding from national and international agencies and I am engaged in several collaborations allowing me to address research projects through a multidisciplinary approach. To date, I have co-authored 69 publications in peer-reviewed international journals (23 as first author and 30 as last and/or corresponding author) and one patent (1714 total citations; H-index 25; WOS, ORCID 0000-0003-0903-5392).
Positions and Employment
1986: Post-graduation training at Neurological Institute "C.Besta" of Milan (Neuropharmacology Lab).
1987-89: Fellowship at Neurological Institute "C.Besta" of Milan. Title of research project: A study of the intracellular antioxidant systems as possible etiopathogenetic cause of Parkinson’s disease".
1989 – 1990: Postgraduate fellow, at the Dept. of Biology, University of Milan, Italy
1990 - 1993: Maternity leave.
1990 – 2001: High scientific officer, tenured position, at the Dept. of Biology, University of Milan, Italy.
June 2000 and February-March 2001: Visiting scientist at the Dept. of Cell Biology, EMBL, Heidelberg, Germany. (Director: Dr. E. Karsenti)
2001 – 2005: Research Associate (Comparative Anatomy and Cytology), University of Milan, Italy.
September 2005 – March 2015: Lecturer of Comparative Anatomy (Assistant professor) and Research group leader, University of Milan, Italy.
April 2015 – October 2018: Associate professor of Comparative Anatomy and Cytology, Department Biosciences, University of Milan, Italy.
October 2017 – present: Coordinator of the master degree in Biology Applied to Research in Biomedicine (University of Milan, Italy)
November 2018 – present: Associate professor of Human Anatomy, Department Biosciences, University of Milan, Italy.
Invited speaker and selected presentations (last 5 years)
SfN (Society for Neuroscience) NEUROSCIENCE 2014, November 15-19, 2014. Washington, (DC) USA. Title of the talk: “Looking at microtubule dysfunction in Parkinson’s disease: from Parkin knockout mice to human iPSCs”.
Gordon Research Conference, “Parkinson’s disease. Emerging research in the etiology and pathogenesis of a complex disease. June 2 – July 3, 2015, New London, NH, USA; Title of the talk: “Parkinson’s disease linked proteins modulate microtubule function: is it the missing piece in the pathogenic pathway?”.
International Meeting “Molecular Neurodegeneration –News and Views in Molecular Neuroscience in Health and Disease”, July 20 – 22, 2015, Delmenhorst, Germany. Title of the talk: “Looking at microtubule dysfunction in toxin- and gene-based models of Parkinson’s disease”.
2nd Meeting in Challenging Organic Synthesis Inspired by Nature - From Natural Products to Drug Discovery, April 4-6, 2016, Madrid, Spain. Title of the talk: “Microtubule defects and targeting agents in experimental models of Parkinson’s disease”.
53° Meeting of the Italian Society of Neuropathology and Clinical Neurobiology, May 18-20, 2017, Padua. Title of the talk: “Alpha-synuclein: from cell biology to pathology”.
6th Mediterranean Neuroscience Society, Malta, June 12-15, 2017, Title of the talk in symposium S11 “Microtubule dysfunction in animal models of Parkinson’s disease”,
Meeting of the GBM Study Group “Molecular Neurobiology”. Dynamics of the Nervous System in Health and Disease, September 20-22, 2018, Marburg (Germany). Title of the talk: “Microtubule dysfunction in Parkinson’s disease: from pure protein to human brain”.
7th Mediterranean Neuroscience Society, Marrakech (Morocco), June 23-27, 2019, Title of the talk in symposium S61 “The interplay between alpha-Synuclein and tubulin/microtubules in health and disease”.
Other experience and Professional Memberships
- Member of SFN Society for Neuroscience; Member of the Italian Society of Neuroscience (SINS); Member of the Italian Society of Human Anatomy and Histology (SIAI); Member of the Italian Society of Neuromorphology (GISN).
- Ad hoc reviewer for BBA Molecular Basis of Disease, Brain Research, Cell Death and Disease, Cellular and Molecular Life Science, Cellular and Molecular Neurobiology, EBioMedicine, European Journal of Pharmacology, Frontiers in Cellular Neuroscience, Neural Plasticity, Neuroscience Letters, European Journal of Pharmacology, PLoS ONE, Scientific Reports.
- Grant reviewer for: MIUR, United States-Israel Binational Science Foundation, Parkinson’ UK.
Research Support (Last 5 years)
- Fondazione Grigioni per il Morbo di Parkinson, 2015-2016. “Mesenchymal stem cells in Progressive Supranuclear Palsy (PSP): evaluation of cytoskeleton structure and stability. Role: Principal Investigator. (45000 euro)
- Sweedish Research Council, 2015-2017. “Patient specific induced pluripotent stem cells to study synucleinopathies”. Role: Collaborator.
- Fondazione Grigioni per il Morbo di Parkinson, 2017. “Role of microtubule dysfuction in Parkinson’s disease and PSP”. Role: Principal Investigator. (30000 euro)
- Fondazione Grigioni per il Morbo di Parkinson, 2017-2019. “Role of microtubule dysfuction in Parkinson’s disease: a study on human brain”. Role: Principal Investigator (60000 euro)
- Università degli Studi di Milano, Program “Linea 2” 2018. “Dissecting the interplay of -synuclein with tubulin/microtubules: from pure proteins to brain”. Role: Principal Investigator (8000 euro)
- Fondazione Grigioni” for Parkinson’s disease, 2020-2021. “Pathogenic mechanisms in Parkinson’s disease and atypical parkinsonisms: a study on human brain”. Role: Principal Investigator (60000 euro/year)
- Marie Skłodowska-Curie Actions - (ITN) Call: H2020- MSCA-ITN-2019 (EJD)" (proposal 860070). “Tuning Tubulin Dynamics and Interactions to Face Neurotoxicity: a Multidisciplinary Approach for Training and Research (Tubintrain)”. Role Partner and supervisor of one Early Stage Researcher (56000 to GC per year, four years)
- Italian Ministry of Education, University and Research (PRIN 2017, project 2017228L3J_002). “Molecular and cellular mechanisms modulating GBA mutation penetrance in Parkinson’s disease”. Role: participant (Funding period 2019-2021, quote funding to GC 10000 euro per year).
I have proven expertise in the field of cell biology of the cytoskeleton and its pivotal role in health and disease. In the first part of my career, I moved from purified proteins to cell cultures and mice models and built a strong experience in studying the microtubule system, its organization and dynamics, in gene- and toxin-based models of Parkinson's disease. I investigated the molecular mechanisms underlying neurodegeneration through multiple approaches ranging from in vitro assays to biochemistry and microscopy analyses in cultured neurons and brain sections by means of over-expression, silencing or incubation with recombinant proteins. In addition, live cell imaging and video-microscopy is used to look at microtubule dynamics and microtubule-dependant processes crucial to maintain neuronal functions, including vesicles recycling and axonal transport. In last years, I became extremely interested in the study of microtubule system and its regulation in human tissues as well as its role in neurodegeneration. My team has been working on a range of human cellular models such as skin fibroblasts, mesenchymal stem cells and induced-Pluripotent Stem Cells, and in human tissues including brain and skin biopsy. We contributed to a novel scenario in which microtubule dysfunction plays a crucial role in the pathogenesis of Parkinson’s disease. The numerous invitations for talks I received remarks the relevance of my work. I have also organized the Symposium S61 “Insights into early cellular dysfunctions leading to synucleinopathies” (7th Mediterranean Neuroscience Society, 2019). Finally, I received funding from national and international agencies and I am engaged in several collaborations allowing me to address research projects through a multidisciplinary approach. To date, I have co-authored 69 publications in peer-reviewed international journals (23 as first author and 30 as last and/or corresponding author) and one patent (1714 total citations; H-index 25; WOS, ORCID 0000-0003-0903-5392).
Positions and Employment
1986: Post-graduation training at Neurological Institute "C.Besta" of Milan (Neuropharmacology Lab).
1987-89: Fellowship at Neurological Institute "C.Besta" of Milan. Title of research project: A study of the intracellular antioxidant systems as possible etiopathogenetic cause of Parkinson’s disease".
1989 – 1990: Postgraduate fellow, at the Dept. of Biology, University of Milan, Italy
1990 - 1993: Maternity leave.
1990 – 2001: High scientific officer, tenured position, at the Dept. of Biology, University of Milan, Italy.
June 2000 and February-March 2001: Visiting scientist at the Dept. of Cell Biology, EMBL, Heidelberg, Germany. (Director: Dr. E. Karsenti)
2001 – 2005: Research Associate (Comparative Anatomy and Cytology), University of Milan, Italy.
September 2005 – March 2015: Lecturer of Comparative Anatomy (Assistant professor) and Research group leader, University of Milan, Italy.
April 2015 – October 2018: Associate professor of Comparative Anatomy and Cytology, Department Biosciences, University of Milan, Italy.
October 2017 – present: Coordinator of the master degree in Biology Applied to Research in Biomedicine (University of Milan, Italy)
November 2018 – present: Associate professor of Human Anatomy, Department Biosciences, University of Milan, Italy.
Invited speaker and selected presentations (last 5 years)
SfN (Society for Neuroscience) NEUROSCIENCE 2014, November 15-19, 2014. Washington, (DC) USA. Title of the talk: “Looking at microtubule dysfunction in Parkinson’s disease: from Parkin knockout mice to human iPSCs”.
Gordon Research Conference, “Parkinson’s disease. Emerging research in the etiology and pathogenesis of a complex disease. June 2 – July 3, 2015, New London, NH, USA; Title of the talk: “Parkinson’s disease linked proteins modulate microtubule function: is it the missing piece in the pathogenic pathway?”.
International Meeting “Molecular Neurodegeneration –News and Views in Molecular Neuroscience in Health and Disease”, July 20 – 22, 2015, Delmenhorst, Germany. Title of the talk: “Looking at microtubule dysfunction in toxin- and gene-based models of Parkinson’s disease”.
2nd Meeting in Challenging Organic Synthesis Inspired by Nature - From Natural Products to Drug Discovery, April 4-6, 2016, Madrid, Spain. Title of the talk: “Microtubule defects and targeting agents in experimental models of Parkinson’s disease”.
53° Meeting of the Italian Society of Neuropathology and Clinical Neurobiology, May 18-20, 2017, Padua. Title of the talk: “Alpha-synuclein: from cell biology to pathology”.
6th Mediterranean Neuroscience Society, Malta, June 12-15, 2017, Title of the talk in symposium S11 “Microtubule dysfunction in animal models of Parkinson’s disease”,
Meeting of the GBM Study Group “Molecular Neurobiology”. Dynamics of the Nervous System in Health and Disease, September 20-22, 2018, Marburg (Germany). Title of the talk: “Microtubule dysfunction in Parkinson’s disease: from pure protein to human brain”.
7th Mediterranean Neuroscience Society, Marrakech (Morocco), June 23-27, 2019, Title of the talk in symposium S61 “The interplay between alpha-Synuclein and tubulin/microtubules in health and disease”.
Other experience and Professional Memberships
- Member of SFN Society for Neuroscience; Member of the Italian Society of Neuroscience (SINS); Member of the Italian Society of Human Anatomy and Histology (SIAI); Member of the Italian Society of Neuromorphology (GISN).
- Ad hoc reviewer for BBA Molecular Basis of Disease, Brain Research, Cell Death and Disease, Cellular and Molecular Life Science, Cellular and Molecular Neurobiology, EBioMedicine, European Journal of Pharmacology, Frontiers in Cellular Neuroscience, Neural Plasticity, Neuroscience Letters, European Journal of Pharmacology, PLoS ONE, Scientific Reports.
- Grant reviewer for: MIUR, United States-Israel Binational Science Foundation, Parkinson’ UK.
Research Support (Last 5 years)
- Fondazione Grigioni per il Morbo di Parkinson, 2015-2016. “Mesenchymal stem cells in Progressive Supranuclear Palsy (PSP): evaluation of cytoskeleton structure and stability. Role: Principal Investigator. (45000 euro)
- Sweedish Research Council, 2015-2017. “Patient specific induced pluripotent stem cells to study synucleinopathies”. Role: Collaborator.
- Fondazione Grigioni per il Morbo di Parkinson, 2017. “Role of microtubule dysfuction in Parkinson’s disease and PSP”. Role: Principal Investigator. (30000 euro)
- Fondazione Grigioni per il Morbo di Parkinson, 2017-2019. “Role of microtubule dysfuction in Parkinson’s disease: a study on human brain”. Role: Principal Investigator (60000 euro)
- Università degli Studi di Milano, Program “Linea 2” 2018. “Dissecting the interplay of -synuclein with tubulin/microtubules: from pure proteins to brain”. Role: Principal Investigator (8000 euro)
- Fondazione Grigioni” for Parkinson’s disease, 2020-2021. “Pathogenic mechanisms in Parkinson’s disease and atypical parkinsonisms: a study on human brain”. Role: Principal Investigator (60000 euro/year)
- Marie Skłodowska-Curie Actions - (ITN) Call: H2020- MSCA-ITN-2019 (EJD)" (proposal 860070). “Tuning Tubulin Dynamics and Interactions to Face Neurotoxicity: a Multidisciplinary Approach for Training and Research (Tubintrain)”. Role Partner and supervisor of one Early Stage Researcher (56000 to GC per year, four years)
- Italian Ministry of Education, University and Research (PRIN 2017, project 2017228L3J_002). “Molecular and cellular mechanisms modulating GBA mutation penetrance in Parkinson’s disease”. Role: participant (Funding period 2019-2021, quote funding to GC 10000 euro per year).