Different effects of anthocyanins and phenolic acids from wild blueberry (Vaccinium angustifolium) on monocytes adhesion to endothelial cells in a TNF-alpha stimulated proinflammatory environment
Articolo
Data di Pubblicazione:
2016
Citazione:
Different effects of anthocyanins and phenolic acids from wild blueberry (Vaccinium angustifolium) on monocytes adhesion to endothelial cells in a TNF-alpha stimulated proinflammatory environment / C. DEL BO', M. Roursgaard, M. Porrini, S. Loft, P. Møller, P. Riso. - In: MOLECULAR NUTRITION & FOOD RESEARCH. - ISSN 1613-4133. - 60:11(2016 Nov), pp. 2355-2366.
Abstract:
SCOPE:
Monocyte adhesion to the vascular endothelium is a crucial step in the early stages of atherogenesis. This study aims to investigate the capacity of an anthocyanin (ACN) and phenolic acid (PA)-rich fraction (RF) of a wild blueberry, single ACNs (cyanidin, malvidin, delphinidin) and related metabolites (protocatechuic, syringic and gallic acid) to counteract monocytes (THP-1) adhesion to endothelial cells (HUVECs) in a tumor necrosis α (TNF-α) mediated pro-inflammatory environment.
METHODS AND RESULTS:
HUVECs were incubated with different concentrations (from 0.01 to 10 μg mL-1 ) of the compounds for 24 h. Labelled monocytic THP-1 cells were added to HUVECs and their adhesion was induced by TNF-α (100 ng mL-1 ). ACN-RF reduced THP-1 adhesion to HUVECs with a maximum effect at 10 μg mL-1 (-33%). PA-RF counteracted THP-1 adhesion at 0.01, 0.1 and 1 μg mL-1 (-45%, -48.7% and -27.6%, respectively), but not at maximum concentration. Supplementation with gallic acid reduced THP-1 adhesion to HUVECs with a maximum effect at 1 μg mL-1 (-29.9%), while malvidin-3-glucoside and syringic acid increased the adhesion. No effect was observed for the other compounds.
CONCLUSION:
These results suggest that ACNs/PA-RF may prevent atherogenesis while the effects of the single ACNs and metabolites are controversial and merit further exploration.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
adhesion; anthocyanins; atherogenesis; cell culture; metabolites; wild blueberry
Elenco autori:
C. DEL BO', M. Roursgaard, M. Porrini, S. Loft, P. Møller, P. Riso
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