Data di Pubblicazione:
2012
Citazione:
Protein modulation in mouse heart under acute and chronic hypoxia / A. Viganò, M. Vasso, A. Caretti, V. Bravatà, L. Terraneo, C. Fania, D. Capitanio, M. Samaja, C. Gelfi, D. Capitanio. - In: PROTEOMICS. - ISSN 1615-9853. - 11:21(2012), pp. 4202-4217.
Abstract:
Exploring cellular mechanisms underlying beneficial and detrimental responses to hypoxia represents the object of the present study. Signaling molecules controlling adaptation to hypoxia (HIF-1 alpha), energy balance (AMPK), mitochondrial biogenesis (PGC-1 alpha), autophagic/apoptotic processes regulation and proteomic dysregulation were assessed. Responses to acute hypoxia (AH) and chronic hypoxia (CH) in mouse heart proteome were detected by 2-D DIGE, mass spectrometry and antigen-antibody reactions. Both in AH and CH, the results indicated a deregulation of proteins related to sarcomere stabilization and muscle contraction. Neither in AH nor in CH the HIF-1 alpha stabilization was observed. In AH, the metabolic adaptation to lack of oxygen was controlled by AMPK activation and sustained by an up-regulation of adenosylhomocysteinase and acetyl-CoA synthetase. AH was characterized by the mitophagic protein Bnip 3 increment. PGC-1 alpha, a master regulator of mitochondrial biogenesis, was down-regulated. CH was characterized by the up-regulation of enzymes involved in antioxidant defense, in aldehyde bio-product detoxification and in misfolded protein degradation. In addition, a general down-regulation of enzymes controlling anaerobic metabolism was observed. After 10 days of hypoxia, cardioprotective molecules were substantially decreased whereas pro-apoptotic molecules increased accompained by down-regulation of specific target proteins
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
AMPK; Animal proteomics; Apoptosis; Autophagy; Heart; Hypoxia
Elenco autori:
A. Viganò, M. Vasso, A. Caretti, V. Bravatà, L. Terraneo, C. Fania, D. Capitanio, M. Samaja, C. Gelfi, D. Capitanio
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