Antioxidant Effect Assessment and Trans Epithelial Analysis of New Hempseed Protein Hydrolysates
Articolo
Data di Pubblicazione:
2023
Citazione:
Antioxidant Effect Assessment and Trans Epithelial Analysis of New Hempseed Protein Hydrolysates / G. Santos-Sánchez, G. Aiello, F. Rivardo, M. Bartolomei, C. Bollati, A. Arnoldi, I. Cruz-Chamorro, C. Lammi. - In: ANTIOXIDANTS. - ISSN 2076-3921. - 12:5(2023 May), pp. 1099.1-1099.19. [10.3390/antiox12051099]
Abstract:
Hempseed (Cannabis sativa) is one of the most promising sources of plant proteins. It con-
tains approximately 24% (w/w) protein, and edestin accounts for approximately 60–80% (w/w) of
its total proteins. In a framework of research aimed at fostering the proteins recovered from the
press cake by-products generated after the extraction of hempseed oil, two hempseed protein hy-
drolysates (HH1 and HH2) were produced at an industrial level using a mixture of different enzymes
from Aspergillus niger, Aspergillus oryzae, and Bacillus licheniformis for different times (5 h and 18 h).
Using a combination of different direct antioxidant tests (DPPH, TEAC, FRAP, and ORAC assays,
respectively), it has been demonstrated that HHs exert potent, direct antioxidant activity. A crucial
feature of bioactive peptides is their intestinal bioavailability; for this reason, in order to solve this
peculiar issue, the ability of HH peptides to be transported by differentiated human intestinal Caco-2
cells has been evaluated. Notably, by using mass spectrometry analysis (HPLC Chip ESI-MS/MS),
the stable peptides transported by intestinal cells have been identified, and dedicated experiments
confirmed that the trans-epithelial transported HH peptide mixtures retain their antioxidant activity,
suggesting that these hempseed hydrolysates may be considered sustainable antioxidant ingredients
to be exploited for further application, i.e., nutraceutical and/or food industries.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
hempseed; bioactive peptides; antioxidant; functional foods; Caco-2 cells; bioavailability
Elenco autori:
G. Santos-Sánchez, G. Aiello, F. Rivardo, M. Bartolomei, C. Bollati, A. Arnoldi, I. Cruz-Chamorro, C. Lammi
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