High-performance liquid chromatographic determination of diclofenac in human plasma after solid-phase extraction
Articolo
Data di Pubblicazione:
2001
Citazione:
High-performance liquid chromatographic determination of diclofenac in human plasma after solid-phase extraction / C. Arcelloni, R. Lanzi, S. Pedercini, G. Molteni, I. Fermo, A Pontiroli, R. Paroni. - In: JOURNAL OF CHROMATOGRAPHY B. BIOMEDICAL SCIENCES AND APPLICATIONS. - ISSN 1387-2273. - 763:1-2(2001 Nov 05), pp. 195-200.
Abstract:
A novel high-performance liquid chromatographic (HPLC) method for the quantification of diclofenac in human plasma was set up. Samples, added with ibuprofen (used as internal standard) were purified by solid-phase extraction using Abselut Nexus cartridges (Varian) not requiring pre-conditioning. Drugs of interest were eluted directly into the autosampler vials and injected. The recovery of diclofenac was 92%, the analysis lasted 7 min with a sensitivity of 5 ng/ml and intra- and inter-day RSDs of 3 and 8%, respectively. The pharmacokinetics of diclofenac after oral and rectal administration in 10 healthy volunteers are reported.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
diclofenac
Elenco autori:
C. Arcelloni, R. Lanzi, S. Pedercini, G. Molteni, I. Fermo, A Pontiroli, R. Paroni
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