Data di Pubblicazione:
2004
Citazione:
Creatinine determination in serum by capillary electrophoresis / R. Paroni, I. Fermo, G. Cighetti, C.A. Ferrero, A. Carobene, F. Ceriotti. - In: ELECTROPHORESIS. - ISSN 0173-0835. - 25:3(2004), pp. 463-468.
Abstract:
Creatinine in human serum was separated in a fused-silica capillary with H3PO4
(75 mmol/L, pH 2.5) as BGE, followed by UV detection at 200 nm. Serum with methylimidazole
added as internal standard was deproteinized with acetonitrile and the
supernatant, after dilution with water was injected at pressure mode. Creatinine and
methylimidazole were baseline-resolved in 6.5 min. Linearity in the 0–880 mmol/L range
gave an r2 0.998, recovery was 102 6 2.8% (n = 6). Enzymatic breakdown with
creatininase confirmed that serum does not interfere. The within-day and betweendays
coefficient of variation (CV) were 2.16 and 2.7%, respectively. The accuracy,
determined for lyophilized samples by isotope dilution gas chromatography-mass
spectrometry was 6 2.0%. The results were compared with HPLC for 32 lyophilized
samples and on 27 serum pools. Capillary electrophoresis, rapid and inexpensive,
seems a promising alternative to high-performance liquid chromatography (HPLC) for
creatinine determination in human serum.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Capillary electrophoresis; Creatinine; High-performance liquid chromatography; Serum
Elenco autori:
R. Paroni, I. Fermo, G. Cighetti, C.A. Ferrero, A. Carobene, F. Ceriotti
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