Bacteriological Quality of Milk in Raw Bovine Bulk Milk in the Selected Milk Collection Centers : Smallholder Dairy Processing Ethiopia

Cross-sectional study was conducted to examine and identify quality of raw bovine milk from in Arsi Zone and East Shewa Zone, in Oromia, Ethiopia. The purpose of the study was to assess hygienic practices during milking, milk collection and bacteriological quality of cow’s milk in Arsi and East Shewa Zones milk collection centers. Total of 246 bacterial isolates were obtained from milk samples taken from Tiyo, Digeluna Tijo, and Lemuna Bilbilo Districts in Arsi Zone and Ada district in East Shewa Zone. Identification of Escherichia coli done using API 20 E biochemical test kit, Gram stain was used to identify staphylococcus aureus, Listeria was identified using Rapid Listeria monocytogenes (RLM), and Brucella identified using Milk ring test (MRT). These included Escherichia coli 19.8%, Listeria monocytogenes 1.2%, Staphylococcus aureus 3.2% and Brucella 3.3%.