Sequencing of the complete gene coding for the GroEL of the Wolbachia of Dirofilaria immitis and expression and purification of the recombinant protein
Articolo
Data di Pubblicazione:
2004
Citazione:
Sequencing of the complete gene coding for the GroEL of the Wolbachia of
Dirofilaria immitis and expression and purification of the recombinant protein / C. Bazzocchi, C. Lecchi, L.H. Kramer, C. Genchi, C. Bandi. - In: PARASSITOLOGIA. - ISSN 0048-2951. - 46:1-2(2004), pp. 307-310.
Abstract:
Wolbachia are intracellular bacteria that infect arthropods and filarial nematodes. These bacteria play an important role in the immunology and pathogenesis of filarial diseases through their proteins and, possibly, other molecules. GroEL is a constitutively expressed bacterial protein; it is highly conserved among bacteria and is involved in the correct folding of newly synthesized proteins. Here we report the production of recombinant GroEL from the Wolbachia of Dirofilaria immitis. Our goal is to test the hypothesis that GroEL is involved in the immunopathology of filariases. The complete groel gene was PCR-amplified, sequenced and cloned into an expression vector. The recombinant GroEL was purified by affinity chromatography by using high-performance liquid chromatography (HPLC).
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Dirofilaria; Filariasis; GroEL; Protein expression; Wolbachia
Elenco autori:
C. Bazzocchi, C. Lecchi, L.H. Kramer, C. Genchi, C. Bandi
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