Release of wheat gluten exorphins A5 and C5 during in vitro gastrointestinal digestion of bread and pasta and their absorption through an in vitro model of intestinal epithelium
Articolo
Data di Pubblicazione:
2015
Citazione:
Release of wheat gluten exorphins A5 and C5 during in vitro gastrointestinal digestion of bread and pasta and their absorption through an in vitro model of intestinal epithelium / M. Stuknyte, M. Maggioni, S. Cattaneo, P. De Luca, A. Fiorilli, A. Ferraretto, I. De Noni. - In: FOOD RESEARCH INTERNATIONAL. - ISSN 0963-9969. - 72(2015 Jun), pp. 208-214.
Abstract:
The release of wheat gluten exorphins (GEs) A5, A4, B5, B4, C5, Gd-7 and dGd-7 during in vitro gastrointestinal digestion (GID) of bread and pasta samples has been investigated by UPLC/ESI–HRMS. The GEs that survived in vitro GID were studied for transport studies across monolayers of 70% Caco-2/30% HT-29 co-culture grown on semi-permeable membranes, used as a model of the human intestinal epithelium. Among the GEs, only the peptides A5 and C5 were detected at the end of in vitro GID of bread and pasta samples. Levels of GEs A5 and C5 were in the range 0.747–2.192 mg kg− 1 and 3.201–6.689 mg kg− 1, respectively. The absorption behavior of A5 and C5 was studied in detail using synthetic peptides. The two peptides were susceptible to the action of Caco-2/HT-29 co-culture peptidases, and 3% and 1% of A5 and C5, respectively, added to the apical chamber were transported to the basolateral chamber after 120 min of incubation, without being hydrolysed. Several hydrolytic fragments of both GEs crossed the co-culture layer and were found in the basolateral chamber. For the first time, these results demonstrate that GEs are released during in vitro GID of real wheat-derived foods and highlight the possibility of transport of the GEs A5 and C5 across the human intestinal mucosa.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Bioavailabilty; Bread; Caco-2/HT-29 co-culture; Gluten exorphins; In vitro digestion; Pasta
Elenco autori:
M. Stuknyte, M. Maggioni, S. Cattaneo, P. De Luca, A. Fiorilli, A. Ferraretto, I. De Noni
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