Data di Pubblicazione:
2013
Citazione:
In search of signals that trigger GPR17
expression in microglia cells / D. Wypych, D. Lecca, M. Fumagalli, M.P. Abbracchio. - In: ACTA NEUROBIOLOGIAE EXPERIMENTALIS. - ISSN 0065-1400. - 73:supplement 1(2013), pp. P8.7.64-P8.7.64. ((Intervento presentato al 11. convegno International congress of the Polish neuroscience society tenutosi a Poznań nel 2013.
Abstract:
After insults microglia cells act by migrating to the site of injury,
phagocyting cell debris and secreting inflammatory mediators,
among which are cytokines, chemokines, cysteinyl leukotrienes
(cysLTs) and purinergic molecules. The recently discovered GPR17
is structurally related to P2Y purinergic and cysLT receptors. Little
is known about its regulation in microglia except that, in animal
models, GPR17 was found in these cells exclusively after ischemic
injury. The aim of this study was to identify in vitro signals that
can trigger GPR17 expression in reactive microglia in vivo. Realtime
PCR showed that in primary rat microglia cells, a low level of
GPR17 can be increased by conditioned medium from oxygen and
glucose deprived neurons or by prolonged deprivation of growth
factors, but not by typical “danger signals” like ATP or cysLT.
Among other known activators of microglia cells, lipopolysaccharide
caused a decrease of GPR17 expression, but GPR17 receptor
agonists and zymosan (a stimulator of phagocytosis) led to its increase
in a time-dependent manner. It is still not known how long,
after induction, GPR17 receptors remain in the membrane and what
function they may play. However preliminary results showed that
GPR17 expression may participate in the acquisition of a detrimental
or a beneficial phenotype of microglia.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
D. Wypych, D. Lecca, M. Fumagalli, M.P. Abbracchio
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