Staphylococcus aureus Efb protein expression in Nicotiana tabacum and immune response to oral administration
Articolo
Data di Pubblicazione:
2013
Citazione:
Staphylococcus aureus Efb protein expression in Nicotiana tabacum and immune response to oral administration / M. Festa, P. Brun, R. Piccinini, I. Castagliuolo, B. Basso, A. Zecconi. - In: RESEARCH IN VETERINARY SCIENCE. - ISSN 0034-5288. - 94:3(2013 Jun), pp. 484-489. [10.1016/j.rvsc.2012.10.012]
Abstract:
Staphylococcus aureus (S. aureus) is one of the most widespread agent of diseases in humans and animals. In dairy cows, S. aureus is the most frequently isolated contagious pathogens in mastitis cases and vaccines are one of the potential tools to control the infections, thus decreasing the use of antibiotics. Among all the virulence factors produced by S. aureus, extra cellular fibrinogen binding protein (Efb) is an important one in the pathogenesis of mastitis. Plants are useful bioreactors to produce antigens and the aim of the study was the production of Efb in two cultivars of Nicotiana tabacum as a mean to produce vaccine against S. aureus in plants. A matrix attachment region (MAR) sequence was inserted near the two borders of transfer-DNA in the transformation vector in the two possible orientations. The presence of MAR elements in the transformation system significantly improved transformation efficiency and Efb protein yield up to a 2% level on total soluble protein (TSP). Mice orally immunized with transgenic lyophilized leaves produced an antigen-specific immune response.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Edible vaccine; MAR; Nicotiana tabacum; Staphylococcus aureus; Vaccine, Efb
Elenco autori:
M. Festa, P. Brun, R. Piccinini, I. Castagliuolo, B. Basso, A. Zecconi
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