COPY NUMBER MODULATION IN AN AUTOSELECTION SYSTEM FOR STABLE PLASMID MAINTENANCE IN SACCHAROMYCES-CEREVISIAE
Articolo
Data di Pubblicazione:
1993
Citazione:
COPY NUMBER MODULATION IN AN AUTOSELECTION SYSTEM FOR STABLE PLASMID MAINTENANCE IN SACCHAROMYCES-CEREVISIAE / C. COMPAGNO, A. TURA, B. RANZI, L. ALBERGHINA, E. MARTEGANI. - In: BIOTECHNOLOGY PROGRESS. - ISSN 8756-7938. - 9:6(1993), pp. 594-599.
Abstract:
Efficient expression of a foreign gene requires a stable vector present at a high number of copies per cell. We have constructed an autoselection system for the stable maintenance of expression vector in the yeast Saccharomyces cerevisiae that uses the fructose 1,6-bisphosphate aldolase gene (FBA1) to stabilize plasmids in cells bearing a disruption of the chromosomal FBA1 gene. This system allowed us to obtain stable production of a reporter heterologous enzyme (Escherichia coli beta-galactosidase) in rich media. By using an inducible promoter to regulate the expression of FBA1 gene, we have also obtained the modulation of plasmid copy number by carbon source.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
C. COMPAGNO, A. TURA, B. RANZI, L. ALBERGHINA, E. MARTEGANI
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