EVALUATION OF AN AUTOMATIC GAS-CHROMATOGRAPHIC SYSTEM FOR THE IDENTIFICATION OF BACTERIAL INFECTIVE AGENTS
Articolo
Data di Pubblicazione:
1989
Citazione:
EVALUATION OF AN AUTOMATIC GAS-CHROMATOGRAPHIC SYSTEM FOR THE IDENTIFICATION OF BACTERIAL INFECTIVE AGENTS / C. ARCELLONI, A. GRIFFINI, R. PARONI, P.A. BONINI. - In: JOURNAL OF AUTOMATIC CHEMISTRY. - ISSN 0142-0453. - 11:5(1989 Sep), pp. 191-200.
Abstract:
The potential clinical application of gas chromatography to
microbial identifcation was evaluated. A completely automated
system, the MIS (Microbial Identification System; Hewlett-
Packard) can analyse and identify pure strains by comparison of
their cellular fatty acids patterns (C9-C2o) with the reference
parameters stored in a library. Three hundred and sixty-seven
strains were tested, comparing the gas chromatographic results
with those obtained by the traditional microbiological methods in
the bacteriology laboratory of our Institute. A standardized
extractive procedure was followed to obtain the fatty acid methyl
esters (FAMEs), but some modifications to the recommended
procedure were introduced in the bacterial growth procedures:
colonies harvested not onlyfrom the recommended growth media but
also from selective media routinely used in the bacteriology
laboratory were successfully examined. These modifications did not
influence the results but improved the ease for the user; good
agreement with the comparison method was observed as far as
identications ofgenus and species are concernedfor 238 cases.
The major advantages of this computerized system are a reduction
in the time required to obtain thejnal results, the elimination of
human errors by using the autosampler and a better inter-laboratory
comparability ofresults owing to a higher degree ofobjectivity. On
the other hand, the limited throughput ofMIS (only 40 samples in
24 h) prevents its use in a large routine laboratory; this technology
is appropriate in emergency cases, in taxonomic studies and as a
confirmatory method.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Gaschromatography; microbial identification test; fatty acids; diagnostic test
Elenco autori:
C. ARCELLONI, A. GRIFFINI, R. PARONI, P.A. BONINI
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