LACK OF CONVERSION OF XANTHINE DEHYDROGENASE TO XANTHINE-OXIDASE DURING WARM RENAL ISCHEMIA
Articolo
Data di Pubblicazione:
1990
Citazione:
LACK OF CONVERSION OF XANTHINE DEHYDROGENASE TO XANTHINE-OXIDASE DURING WARM RENAL ISCHEMIA / G. CIGHETTI, M. DELPUPPO, R. PARONI, M.G. KIENLE. - In: FEBS LETTERS. - ISSN 0014-5793. - 274:1-2(1990 Nov 12), pp. 82-84.
Abstract:
Irreversible transformation of xanthine dehydrogenase (XDH) to xanthine oxidase (XO) during ischemia was determined measuring XDH and
total enzyme activity in kidneys before and after 60 min ofclamp ofthe renal pedicle. Tissue levels ofadenine nucleotides, xanthine and hypoxanthine
were used as indicators of ischemia. After 60 min of clamping, ATP levels decreased by 72/. with respect to controls whereas xanthine and
hypoxanthine progressively reached tissue concentrations of 732+49 and 979+ l5 nmol'g tissue 1, respectively. Both total and XDH activities
in ischemic kidneys (30+ 15 and l9* I nmol'min I'g tissue-r) were significantly lower than in controls when expressed on a tissue weight basis.
The fraction of enzyme in the XDH form was however unchanged indicating that the reduction of the nucleotide pool is not accompanied by
induction ofthe type-O activity ofxanthine oxidase.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Xanthine oxidase; Xanthine dehydrogenase; Renal ischemia; Adenine nucleotide; Rat kidney; HPLC
Elenco autori:
G. CIGHETTI, M. DELPUPPO, R. PARONI, M.G. KIENLE
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