Ketoconazole-mediated growth inhibition in Botrytis cinerea and Saccharomyces cerevisiae

Minimum inhibitory concentrations (MIC) for Botrytis cinerea and Saccharomyces cerevisiae with ketoconazole were 10 μg ml- 1 and 20 μg ml- 1, respectively. The time-course of inhibition with treatment at the MIC was examined for each organism and the sterol profile determined. Botrytis cinerea initially accumulated 24-methylene-24(25)-dihydrolanosterol and then obtusifolione during growth arrest. Saccharomyces cerevisiae initially accumulated lanosterol with subsequent production of 3,6 diol, just prior to growth arrest.