MmNeu3 sialidase over-expression in C2C12 myoblasts delayed muscle differentiation and induced hypertrophic myotubes formation
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Data di Pubblicazione:
2011
Citazione:
MmNeu3 sialidase over-expression in C2C12 myoblasts delayed muscle differentiation and induced hypertrophic myotubes formation / L. Anastasia, L. Dileo, C. Tringali, E. Monti, G. Tettamanti, B. Venerando, N. Papini. ((Intervento presentato al 9. convegno International Symposium on: "Biochemical Roles of Eukaryotic Cell Surface Macromolecules" tenutosi a Trivandrum nel 2011.
Abstract:
Recently we have begun to investigated the
role of NEU3 in skeletal muscle
differentiation, in particular we have
demonstrated that in C2C12 myoblasts downregulation
of NEU3 totally inhibits cell
capability to differentiate and causes an high
responsiveness of myoblasts to apoptosis. It
has been shown also that the increase of GM3
levels caused epidermal growth factor
receptor inhibition and down-regulation. In
order to support the role of NEU3 in skeletal
muscle differentiation we decided to stably
overespress MmNEU3 in C2C12 cells by a
lentiviral vector and to investigate the
behaviour of the differentiation process. We
found that NEU3 over-expressing C2C12 cells
(a) delay differentiation and continue to
proliferate for at least two more days than
control cells; (b) when they enter the
differentiation process, complete it very
quickly; (c) form hypertrophic myotubes; (d)
show an increased activity of the EGF-R
signalling. Thus, this work further supports
the central role of NEU3 as key modulator in skeletal muscle differentiation
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Keywords:
Sialidase Neu3 ; Myoblasts ; Differentiation
Elenco autori:
L. Anastasia, L. Dileo, C. Tringali, E. Monti, G. Tettamanti, B. Venerando, N. Papini
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