Data di Pubblicazione:
2023
Citazione:
Protocol to evaluate the impact of murine MCT1-deficient CD8+ T cells on adipogenesis / C. Macchi, A. Moregola, G.D. Norata, M. Ruscica. - In: STAR PROTOCOLS. - ISSN 2666-1667. - 4:2(2023 Jun 16), pp. 102301.1-102301.12. [10.1016/j.xpro.2023.102301]
Abstract:
The infiltration of activated T cells, such as CD8+ effector, in metabolic tissues plays a crucial role for the initiation and propagation of obesity-induced inflammation. Given the pivotal role of lactate transporter monocarboxylate transporter 1 (MCT1) in immune cell activation, we present a protocol for the isolation and activation of CD8+ T lymphocytes selectively lacking MCT1. We describe steps for the induction of adipocyte differentiation, CD8+ T isolation and activation, and adipocyte-CD8+ T cell co-culture. We then detail qPCR analysis on differentiated adipocytes. For complete details on the use and execution of this protocol, please refer to Macchi et al.1.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Cell Biology; Cell culture; Cell isolation; Flow Cytometry/Mass Cytometry; Metabolism; Model Organisms; Molecular Biology
Elenco autori:
C. Macchi, A. Moregola, G.D. Norata, M. Ruscica
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