Data di Pubblicazione:
2019
Citazione:
Inflammatory role of extracellular sphingolipids in Cystic Fibrosis / A. Zulueta, V. Peli, M. Dei Cas, M. Colombo, R. Paroni, M. Falleni, A. Baisi, V. Bollati, R. Chiaramonte, E. Del Favero, R. Ghidoni, A. Caretti. - In: THE INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY. - ISSN 1357-2725. - 116:(2019). [10.1016/j.biocel.2019.105622]
Abstract:
Ceramide is emerging as one of the players of inflammation in lung diseases. However, data on its inflammatory role in Cystic Fibrosis (CF) as part of the extracellular machinery driven by lung mesenchymal stem cells (MSCs)-derived extracellular vesicles (EVs) are missing. We obtained an in vitro model of CF-MSC by treating control human lung MSCs with a specific CFTR inhibitor. We characterized EVs populations derived from MSCs (ctr EVs) and CF-MSCs (CF-EVs) and analyzed their sphingolipid profile by LC-MS/MS. To evaluate their immunomodulatory function, we treated an in vitro human model of CF, with both EVs populations. Our data show that the two EVs populations differ for the average size, amount, and rate of uptake. CF-EVs display higher ceramide and dihydroceramide accumulation as compared to control EVs, suggesting the involvement of the de novo biosynthesis pathway in the parental CF-MSCs. Higher sphingomyelinase activity in CF-MSCs, driven by inflammation-induced ceramide accumulation, sustains the exocytosis of vesicles that export new formed pro-inflammatory ceramide. Our results suggest that CFTR dysfunction associates with an enhanced sphingolipid metabolism leading to the release of EVs that export the excess of pro-inflammatory Cer to the recipient cells, thus contributing to maintain the unresolved inflammatory status of CF.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Ceramide; Extracellular vesicles; Inflammation; Mesenchymal stem cells; Sphingolipids
Elenco autori:
A. Zulueta, V. Peli, M. Dei Cas, M. Colombo, R. Paroni, M. Falleni, A. Baisi, V. Bollati, R. Chiaramonte, E. Del Favero, R. Ghidoni, A. Caretti
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