Simultaneous Determination of the Main Peanut Allergens in Foods Using Disposable Amperometric Magnetic Beads-Based Immunosensing Platforms
Articolo
Data di Pubblicazione:
2016
Citazione:
Simultaneous Determination of the Main Peanut Allergens in Foods Using Disposable Amperometric Magnetic Beads-Based Immunosensing Platforms / V. Ruiz-Valdepeñas Montiel, R. Torrente-Rodríguez, S. Campuzano, A. Pellicanò, Á. Reviejo, M. Cosio, J. Pingarrón. - In: CHEMOSENSORS. - ISSN 2227-9040. - 4:3(2016 Jun 28), p. 11.
Abstract:
In this work, a novel magnetic beads (MBs)-based immunosensing approach for the rapid
and simultaneous determination of the main peanut allergenic proteins (Ara h 1 and Ara h 2)
is reported. It involves the use of sandwich-type immunoassays using selective capture and
detector antibodies and carboxylic acid-modified magnetic beads (HOOC-MBs). Amperometric
detection at 0.20 V was performed using dual screen-printed carbon electrodes (SPdCEs) and the
H2O2/hydroquinone (HQ) system. This methodology exhibits high sensitivity and selectivity for the
target proteins providing detection limits of 18.0 and 0.07 ng/mL for Ara h 1 and Ara h 2, respectively,
with an assay time of only 2 h. The usefulness of the approach was evaluated by detecting the
endogenous content of both allergenic proteins in different food extracts as well as trace amounts
of peanut allergen (0.0001% or 1.0 mg/kg) in wheat flour spiked samples. The developed platform
provides better Low detection limits (LODs) in shorter assay times than those claimed for the allergen
specific commercial ELISA kits using the same immunoreagents and quantitative information on
individual food allergen levels. Moreover, the flexibility of the methodology makes it readily translate
to the detection of other food-allergens.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Ara h 1; Ara h 2; dual determination; magnetic beads; SPdCEs; amperometric
immunosensor; food extracts
Elenco autori:
V. Ruiz-Valdepeñas Montiel, R. Torrente-Rodríguez, S. Campuzano, A. Pellicanò, Á. Reviejo, M. Cosio, J. Pingarrón
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