Galactose utilization sheds new light on sugar metabolism in the sequenced strain Dekkera bruxellensis CBS 2499
Articolo
Data di Pubblicazione:
2015
Citazione:
Galactose utilization sheds new light on sugar metabolism in the sequenced strain Dekkera bruxellensis CBS 2499 / M. Moktaduzzaman, S. Galafassi, C. Capusoni, I. Vigentini, Z. Ling, J. Piškur, C. Compagno. - In: FEMS YEAST RESEARCH. - ISSN 1567-1356. - 15:2(2015 Mar), pp. fou009.1-fou009.9. [10.1093/femsyr/fou009]
Abstract:
Dekkera bruxellensis and Saccharomyces cerevisiae are considered two phylogenetically distant relatives, but they share several industrial relevant traits such as the ability to produce ethanol under aerobic conditions (Crabtree effect), high tolerance towards ethanol and acids, and ability to grow without oxygen. Beside a huge adaptability, D. bruxellensis exhibits a broader spectrum in utilization of carbon and nitrogen sources in comparison to S. cerevisiae. With the aim to better characterize its carbon source metabolism and regulation, the usage of galactose and the role that glucose plays on sugar metabolism were investigated in D. bruxellensis CBS 2499. The results indicate that in this yeast galactose is a non-fermentable carbon source, in contrast to S. cerevisiae that can ferment it. In particular, its metabolism is affected by the nitrogen source. Interestingly, D. bruxellensis CBS 2499 exhibits the 'short-term Crabtree effect', and the expression of genes involved in galactose utilization and in respiratory metabolism is repressed by glucose, similarly to what occurs in S. cerevisiae.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
carbon metabolism; nitrogen metabolism; glucose repression; acetic acid production; ethanol production
Elenco autori:
M. Moktaduzzaman, S. Galafassi, C. Capusoni, I. Vigentini, Z. Ling, J. Piškur, C. Compagno
Link alla scheda completa:
Link al Full Text: