Identification of endogenous genetic elements in Oenococcus oeni for the development of novel molecular tools
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Data di Pubblicazione:
2021
Citazione:
Identification of endogenous genetic elements in Oenococcus oeni for the development of novel molecular tools / A. Di Canito, R. Foschino, G. Felis, I. Vigentini. ((Intervento presentato al 6. convegno Microbial Diversity tenutosi a On line : 14 to 15 December nel 2021.
Abstract:
Oenococcus oeni is a lactic acid bacterium associated to the wine-microbiota. It is considered
the main actor of the malolactic fermentation in wine, contributing to the complexity of the
final product. Besides its contribution to the winemaking process, this species is able to
produce interesting aromas and functional compounds (i.e., melatonin). Due to the lack of
efficient tools for its genetic manipulation, the molecular mechanisms underlying its capability
to survive in the oenological environment and to develop compounds of interest are still
poorly investigated. Several studies suggest that O. oeni genetic variability is associated to
genetic elements for horizontal transfer, both natural plasmids and oenophages, which are
known to contain adaptive advantage traits. For this reason, the aim of this work is the
identification of genetic elements for the construction of new molecular tools exploitable in
genetic functional studies and potential future biotechnological applications.
Seventy-one O. oeni strains (isolated from Valtellina, Aosta Valley and Valpolicella wines or
wineries) were investigated for the presence of these genetic elements. Three natural plasmids
(pOeA, pOeB and pOeC) showing a high percentage of similarity with the well-known O. oeni
pOENI-1 and pOENI-1v2 plasmids were isolated and sequenced. By combining the
amplification of five target genes (tauE, oye, oye_parB, repAlys), nine strains were found to
putatively contain phages. In particular, the genetic analysis of the four groups of integrases
(A, B, C or D) showed that four strains could belong to the group A, one to the group C, while
one responded positively both to group A and C. Finally, phylogenetic analyses using the lys
gene sequences were performed to evaluate the evolutionary relationship of the putative
phages. In conclusion, the selected strains and identified genetic elements represent a valuable
resource to shed light in the O. oeni genetics and metabolism.
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
A. Di Canito, R. Foschino, G. Felis, I. Vigentini
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