Milk safety and quality and their associated risks pose a major concern worldwide regarding not only the relative economical losses but also the potential danger to consumer's health. Customer's confidence in the integrity of the milk supply could be hampered by inappropriate milk safety measures. A lack of measures and reliable assays to evaluate and maintain a good control of milk characteristics may affect the milk industry economy started from animal welfare and shatter consumer confidence.It is imperative to create and to establish fast and reliable analytical methods that allow a good and rapid analysis of milk and dairy products during the whole food chain. The project is designed to provide a rapid, economic,and alternative approach to the control of the safety of milk through the entire supply chain and with regard to the biotic substances both for those abiotic, including allergens.The rapid systems will be developed with established integrated omics techniques,in particular genomics,proteomics,peptidomics and metabolomics,in order to provide economic and fast systems for breeder-dairy producers for the protection and enhancement of their products. Milk and its derivatives constitute fundamental nutrients for all age groups;milk is a real functional food, and may be subject to microbial contamination or alteration of the basic characteristics thus representing a potential danger to public health. Safety and quality of milk is not only given by the microbial presence,but also by the presence of alterations in protein structure that can thus affect the functional characteristics of the food itself; so also in the bulk milk contaminants may be present such as xenobiotics with effects, which then also affect the dairy industry.However,there is still the need of rapid methods for the identification of these compounds in the range of concentrations of interest, and especially of valid indicators which guarantee the farmer on one side and the other the product to the consumer milk as functional food preserved in all its peculiarities.The omics sciences,making use of technological platforms with very high sensitivity and resolution exceed the limits of identification and quantification of the currently available traditional techniques.In this approach, omics can give a valuable contribution thanks to the direct study of the functional product, proteins, so that they become a real tool can be used as indicator of safety and quality throughout the supply chain.The structural and accurate determination of the proteins that make up a specific sample there may in fact provide useful information to know not only the composition of the microbiological profile but also the chemical and physical conditions through which the product of the dairy industry has come in contact. Proteomics,metaproteomics and metabololics can represent a powerful tool to address this issue,due to their proven excellent quantitative and qualitative performance in protein analysis. This field is rapidly emerging and there are many applications of proteomics in the past few years in milk science from food processing, allergies, and possible contaminants like bacteria, fungi, and other pathogens.In particular,the study of microbiota with metaproteomics approach will give new insights in this field. This project will address new rapid systems for the monitoring of biotic and abiotic substances in milk to improve also dairy products along the food chain.In particular, the project will be able to correlate the microbial ecosystem in milk and dairy products to the environment and to animal health. These will be realized through the integrations of high throughput molecular approaches (metagenomics-metaproteomics). Moreover, it is very important to explore how industrial processing procedures, which are used to guarantee microbiological safety and/or extend shelf life, alter the level of allergenicity of milk products, incidence of which is increasing in newest gen