Smc5/6 functions with Sgs1-Top3-Rmi1 to complete chromosome replication at natural pause sites
Articolo
Data di Pubblicazione:
2021
Citazione:
Smc5/6 functions with Sgs1-Top3-Rmi1 to complete chromosome replication at natural pause sites / S. Agashe, C.R. Joseph, T.A.C. Reyes, D. Menolfi, M. Giannattasio, A. Waizenegger, B. Szakal, D. Branzei. - In: NATURE COMMUNICATIONS. - ISSN 2041-1723. - 12:1(2021 Apr), pp. 2111.1-2111.15. [10.1038/s41467-021-22217-w]
Abstract:
Smc5/6 is essential for genome structural integrity by yet unknown mechanisms. Here we find that Smc5/6 co-localizes with the DNA crossed-strand processing complex Sgs1-Top3-Rmi1 (STR) at genomic regions known as natural pausing sites (NPSs) where it facilitates Top3 retention. Individual depletions of STR subunits and Smc5/6 cause similar accumulation of joint molecules (JMs) composed of reversed forks, double Holliday Junctions and hemicatenanes, indicative of Smc5/6 regulating Sgs1 and Top3 DNA processing activities. We isolate an intra-allelic suppressor of smc6-56 proficient in Top3 retention but affected in pathways that act complementarily with Sgs1 and Top3 to resolve JMs arising at replication termination. Upon replication stress, the smc6-56 suppressor requires STR and Mus81-Mms4 functions for recovery, but not Srs2 and Mph1 helicases that prevent maturation of recombination intermediates. Thus, Smc5/6 functions jointly with Top3 and STR to mediate replication completion and influences the function of other DNA crossed-strand processing enzymes at NPSs.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Cell Cycle Proteins; DNA Repair; DNA Replication; DNA-Binding Proteins; Endonucleases; Flap Endonucleases; Genome, Fungal; RecQ Helicases; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins
Elenco autori:
S. Agashe, C.R. Joseph, T.A.C. Reyes, D. Menolfi, M. Giannattasio, A. Waizenegger, B. Szakal, D. Branzei
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