Construction of human ghrelin receptor (hGHS-R1a) model using a fragmental prediction approach and validation through docking analysis
Articolo
Data di Pubblicazione:
2006
Citazione:
Construction of human ghrelin receptor (hGHS-R1a) model using a fragmental prediction approach and validation through docking analysis / A. Pedretti, M. Villa, M. Pallavicini, E. Valoti, G. Vistoli. - In: JOURNAL OF MEDICINAL CHEMISTRY. - ISSN 0022-2623. - 49:11(2006), pp. 3077-3085. [10.1021/jm058053k]
Abstract:
The objective of this study was to investigate the reliability of a fragmental approach to build a full-length model of the human ghrelin receptor (hGHS-R1a) in its open state. The soundness of the model was verified by docking the tetrapeptide Gly-Ser-Ser(n-octanoyl)-Phe-NH2, which represents the ghrelin active core, and a dataset of 35 peptidomimetic GH secretagogues taken from literature. Docking results confirm the relevance of two distinct subpockets: a polar cavity bearing the key residues involved in receptor activation and an arom./apolar subpocket, which plays a crucial role in detg. the high constitutive activity of hGHS-R1a. The docking scores of both subpockets are in remarkable agreement with biol. data, emphasizing that the model can be used to predict the activity of novel ligands. Moreover, the subpocket selectivity of peptidomimetic GHSs suggests a cooperative role of the arom./apolar subpocket. Taken globally, the results highlight the potential of the fragmental approach to build improved models for any GPCR
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Human; Molecular association; Simulation and Modeling (construction of human ghrelin receptor (hGHS-R1a) model using fragmental prediction approach and validation through docking anal.); Growth hormone secretagogue receptors Role: BSU (Biological study, unclassified), BIOL (Biological study) (construction of human ghrelin receptor (hGHS-R1a) model using fragmental prediction approach and validation through docking anal.); Structure-activity relationship (ghrelin receptor-binding; construction of human ghrelin receptor (hGHS-R1a) model using fragmental prediction approach and validation through docking anal.); Conformation (protein; construction of human ghrelin receptor (hGHS-R1a) model using fragmental prediction approach and validation through docking anal.)
Elenco autori:
A. Pedretti, M. Villa, M. Pallavicini, E. Valoti, G. Vistoli
Link alla scheda completa: