Merkel Cell Polyomavirus Is Associated with Anal Infections in Men Who Have Sex with Men
Academic Article
Publication Date:
2019
Citation:
Merkel Cell Polyomavirus Is Associated with Anal Infections in Men Who Have Sex with Men / N. Zanotta, S. Delbue, L. Signorini, S. Villani, S. D'Alessandro, G. Campisciano, C. Colli, F. De Seta, P. Ferrante, M. Comar. - In: MICROORGANISMS. - ISSN 2076-2607. - 7:2(2019 Feb 19), pp. 54.1-54.12. [10.3390/microorganisms7020054]
abstract:
BACKGROUND:
Viral infections of the anal/rectal tract of men who have sex with men (MSM) have been poorly studied.
METHODS:
In total, 158 swab samples (81 anal/rectal, 65 throat/oral and 12 urethral) were collected from 126 MSM. DNA was isolated and subjected to real-time PCR assays for the detection of the sexually transmitted (ST) pathogens Chlamydia trachomatis, Neisseria gonorrhoeae and Mycoplasmas ssp, human papillomavirus (HPV) and six human polyomaviruses (HPyVs; JCPyV, BKPyV, Merkel cell PyV⁻MCPyV-, HPyV-6, HPyV-7 and HPyV-9).
RESULTS:
C. trachomatis (31/126, 24.6%) and M. genitalium (30/126, 23.8%) were the most frequently detected ST pathogens. Thirty-one/126 (24.6%) patients were positive for at least one HPyV. The significantly (p < 0.05) prevalent HPyV in the anal tract was MCPyV, which was amplified in 27/81 (33.3%) samples, followed by HPyV-6, which was amplified in 6/81 (7.4%) swabs. Coinfections with MCPyV and C. trachomatis or Mycoplasmas were found in 4/21 (19.0%) and 5/21 (23.8%) anal/rectal swabs, respectively. Three/4 MCPyV-C. trachomatis coinfected patients were symptomatic.
CONCLUSIONS:
Based on the high prevalence of MCPyV in the anal/rectal swabs from MSM patients and on the well-known oncogenic properties of MCPyV, sexual transmission and possible involvement of HPyVs in the pathogenesis of diseases of the anal canal should be further studied.
Viral infections of the anal/rectal tract of men who have sex with men (MSM) have been poorly studied.
METHODS:
In total, 158 swab samples (81 anal/rectal, 65 throat/oral and 12 urethral) were collected from 126 MSM. DNA was isolated and subjected to real-time PCR assays for the detection of the sexually transmitted (ST) pathogens Chlamydia trachomatis, Neisseria gonorrhoeae and Mycoplasmas ssp, human papillomavirus (HPV) and six human polyomaviruses (HPyVs; JCPyV, BKPyV, Merkel cell PyV⁻MCPyV-, HPyV-6, HPyV-7 and HPyV-9).
RESULTS:
C. trachomatis (31/126, 24.6%) and M. genitalium (30/126, 23.8%) were the most frequently detected ST pathogens. Thirty-one/126 (24.6%) patients were positive for at least one HPyV. The significantly (p < 0.05) prevalent HPyV in the anal tract was MCPyV, which was amplified in 27/81 (33.3%) samples, followed by HPyV-6, which was amplified in 6/81 (7.4%) swabs. Coinfections with MCPyV and C. trachomatis or Mycoplasmas were found in 4/21 (19.0%) and 5/21 (23.8%) anal/rectal swabs, respectively. Three/4 MCPyV-C. trachomatis coinfected patients were symptomatic.
CONCLUSIONS:
Based on the high prevalence of MCPyV in the anal/rectal swabs from MSM patients and on the well-known oncogenic properties of MCPyV, sexual transmission and possible involvement of HPyVs in the pathogenesis of diseases of the anal canal should be further studied.
IRIS type:
01 - Articolo su periodico
Keywords:
Merkel cell polyomavirus (MCPyV); men who have sex with men (MSM); sexually transmitted infection (STI)
List of contributors:
N. Zanotta, S. Delbue, L. Signorini, S. Villani, S. D'Alessandro, G. Campisciano, C. Colli, F. De Seta, P. Ferrante, M. Comar
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