Enzymatic Synthesis of Valuable Bioactive Compounds

sustainable and convenient, one-pot two-enzyme method for the glucosylation of arylalkyl alcohols was developed (Scheme 1). The reaction scheme was based on a transrutinosylation catalyzed by a rutinosidase from A. niger using the cheap and commercially available flavonoid rutin as glycosyl donor, followed by a selective ‘trimming’ of the rutinoside unit, catalyzed by a rhamnosidase from A. terreus. Both these enzymes were available to us as heterologous proteins produced by a recombinant strain of P. pastoris. This process allowed the facile preparation of several natural bioactive glucosides, which could be isolated in up to 80% yield without the need of silica-gel chromatography.