Secretion of basic fibroblast growth factor (FGF-2) by WEHI-3B myelomonocitic leukemia cells
Articolo
Data di Pubblicazione:
2002
Citazione:
Secretion of basic fibroblast growth factor (FGF-2) by WEHI-3B myelomonocitic leukemia cells / A. Pessina, G. Gagliardi, C. Croera, P. Foti, C. Dassi, P. Brambilla, M.G. Neri. - In: GROWTH FACTORS. - ISSN 0897-7194. - 20:3(2002), pp. 121-129.
Abstract:
In order to investigate the role of Fibroblast Growth Factors in hematopoietic cells, we studied the expression of FGF-1, FGF-2, FGF-3, FGF-4, FGF-5 and FGF-6 mRNAs both in murine myelomonocytic leukemia WEHI-3B and in a murine stromal cell line SR-4987. Secretion of FGF-2 in the cell culture supernatant was also studied. Expression of mRNA encoding for the above-mentioned FGFs was analyzed by RT-PCR. The production of FGF-2 in the conditioned media of WEHI-3B and SR-4987 cell cultures was evaluated by techniques of affinity chromatography, chromatofocusing and immunoblotting. The biological activity of FGF-2 was checked on SR-4987 cells by a agar clonogenic assay. In both cell lines mRNA was found encoding for FGF-1, FGF-2 and FGF-6 and WEHI-3B cells express also mRNA for FGF-3 (int-2) and FGF-4 (K-FGF/hst). Furthermore, supernatant from WEHI-3B cells was found to stimulate dramatically the agar clonogenicity of SR-4987 cells which have a very poor basal capacity for growth in agar. The clonogenic activity of WEHI-3B conditioned medium is due to FGF-2 secreted into cell culture supernatant whereas SR-4987 cells, although express FGF-2 mRNA, do not seem able to secrete this factor. The expression in myeloid leukemia cells of oncogene-related factors such as FGF-3, FGF-4 and FGF-6 together with the secretion of FGF-2 able to support a positive regulation of bone marrow stromal cells function suggest that FGFs may have an important role in sustaining the leukemogenic process and related disorders.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Anchorage dependency; FGF-2; Fibroblast growth factors; SR-4987 stromal cells; WEHI-3B myelomonocytic leukemia
Elenco autori:
A. Pessina, G. Gagliardi, C. Croera, P. Foti, C. Dassi, P. Brambilla, M.G. Neri
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