Mass Spectrometry-based Label-free Quantitative Proteomics to Study the Effect of 3PO Drug at Cellular Level
Articolo
Data di Pubblicazione:
2019
Citazione:
Mass Spectrometry-based Label-free Quantitative Proteomics to Study the Effect of 3PO Drug at Cellular Level / S.B. Nukala, G. Baron, G. Aldini, M. Carini, A. D'Amato. - In: ACS MEDICINAL CHEMISTRY LETTERS. - ISSN 1948-5875. - 10:4 (special issue)(2019 Apr), pp. 577-583.
Abstract:
Human endothelial cells (ECs) have been employed to monitor the protein changes induced by [3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one] (3PO), a compound able to inhibit the glycolytic flux partially and transiently and to reduce pathological angiogenesis in a variety of disease models. Normal and TNFα induced inflamed ECs were incubated with and without 3PO at a concentration (20 μM) able to inhibit cell proliferation without cell death. At the end of the incubation period, samples were submitted to the following steps: (a) whole protein extraction, reduction, alkylation, and digestion by trypsin; (b) peptide separation by nano-LC-MS/MS analysis using a high-resolution mass spectrometer; (c) data analysis including protein identification, quantification, and statistical analysis. An altered protein expression profiling in combination with protein network analysis was employed by using a mass spectrometry-based label-free quantification approach to explore the underlying mechanisms of 3PO at cellular level.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
3PO; cell pathway modulation; inflammation; mass spectrometry; PFKFB3 inhibitor; Quantitative proteomics; Biochemistry; Drug Discovery3003 Pharmaceutical Science; Organic Chemistry
Elenco autori:
S.B. Nukala, G. Baron, G. Aldini, M. Carini, A. D'Amato
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