Amplified in situ hybridization with peptide nucleic acid probes for differentiation of Mycobacterium tuberculosis complex and nontuberculous Mycobacterium species on formalin-fixed, paraffin-embedded archival biopsy and autopsy samples
Articolo
Data di Pubblicazione:
2001
Citazione:
Amplified in situ hybridization with peptide nucleic acid probes for differentiation of Mycobacterium tuberculosis complex and nontuberculous Mycobacterium species on formalin-fixed, paraffin-embedded archival biopsy and autopsy samples / P. Zerbi, A. Schonau, S. Bonetto, A. Gori, G. Costanzi, P.G. Duca, L. Vago. - In: AMERICAN JOURNAL OF CLINICAL PATHOLOGY. - ISSN 0002-9173. - 116:5(2001 Nov), pp. 770-775. [10.1309/K7XC-0VXF-KE10-0UFH]
Abstract:
The aim of this study was to evaluate sensitivity and specificity of in situ hybridization (ISH) using peptide nucleic acid (PNA) probes and tyramide-based
amplification for the differentiation between Mycobacterium tuberculosis (MTB) and mycobacteria other than tuberculosis (MOTT) on formalin-fixed,
paraffin-embedded tissue samples. We performed ISH simultaneously with both
probes on 86 specimens from different organs: 70 obtained at autopsy and 16 by
biopsy, all with a histologic evidence of mycobacterial infection confirmed by
Ziehl-Neelsen-positive staining. Taking culture as the "gold standard," the
sensitivity and the specificity of the MTB probe were 100% (41/41) and 95%
(38/40), respectively. In only 2 cases ISH failed to identify mycobacteria.
Culture results were not available in 3 cases. We propose ISH as a relatively
simple and rapid method to differentiate mycobacteria on formalin-fixed,
paraffin-embedded specimens (it is more specific than usual histologic stains)
and as an alternative to polymerase chain reaction, allowing the morphologic
evaluation of positive bacilli.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
P. Zerbi, A. Schonau, S. Bonetto, A. Gori, G. Costanzi, P.G. Duca, L. Vago
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