Impact of ConcanavalinA affinity in the intracellular fate of Protein Corona on Glucosamine Au nanoparticles
Articolo
Data di Pubblicazione:
2018
Citazione:
Impact of ConcanavalinA affinity in the intracellular fate of Protein Corona on Glucosamine Au nanoparticles / D. Di Silvio, A. Silvestri, L. Lay, L. Polito, S.E. Moya. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 8:1(2018 Jun 13).
Abstract:
Biological fate and toxicity of nanoparticles (NPs) are connected to the interaction between NPs and
the protein corona (PC) spontaneously forming around NPs in biological matrixes. PC is a dynamic
entity that confers biological identity to NPs. In this work, fluorescence cross-correlation spectroscopy
(FCCS) is used to study the impact of specific interactions between the NP surface and proteins on
the intracellular fate of PC. The stability of the PC formed around glucosamide-functionalized Au-NPs
from ConcanavalinA (ConA) or Bovine Serum Albumin (BSA) is characterized by FCCS. The NPs show
higher affinity for ConA and competitive assays show that ConA easily exchanges BSA. A549 cells are
exposed to glucosamide-functionalized Au-NPs with preformed ConA and BSA PCs. Intracellularly the
frequency of cross-correlation for Au NPs with ConA PC remains constant to a 70% value until 24 h while
for BSA it decreases to a 15% during the same period. FCCS measurements in several locations in the
cell point out a different level of aggregation for the NPs with either ConA or BSA PCs. Our results show
that the affinity of NPs functionalized with a ligand with affinity for a specific protein in bulk is retained
intracellularly influencing NP fate and translocation.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
D. Di Silvio, A. Silvestri, L. Lay, L. Polito, S.E. Moya
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