PPARalpha inhibits vascular smooth muscle cell proliferation underlying intimal hyperplasia by inducing the tumor suppressor gene p16INK4a
Articolo
Data di Pubblicazione:
2005
Citazione:
PPARalpha inhibits vascular smooth muscle cell proliferation underlying intimal hyperplasia by inducing the tumor suppressor gene p16INK4a / F. Gizard, C. Amant, O. Barbier, S. Bellosta, R. Robillard, F. Percevault, H. Sevestre, P. Krimpenfort, A. Corsini, J. Rochette, C. Glineur, J.C. Fruchart, G. Torpier, B. Staels. - In: THE JOURNAL OF CLINICAL INVESTIGATION. - ISSN 0021-9738. - 115:11(2005), pp. 3228-3238. [10.1172/JCI22756]
Abstract:
Vascular SMC proliferation is a crucial event in occlusive cardiovascular diseases. PPAR alpha is a nuclear receptor controlling lipid metabolism and inflammation, but its role in the regulation of SMC growth remains to be established. Here, we show that PPARa. controls SMC cell-cycle progression at the G(1)/S transition by targeting the cyclin-dependent kinase inhibitor and tumor suppressor p16(INK4a) (p16), resulting in an inhibition of retinoblastoma protein phosphorylation. PPARa activates p16 gene transcription by both binding to a canonical PPAR-response element and interacting with the transcription factor Sp1 at specific proximal Sp1-binding sites of the p16 promoter. In a carotid arterial-injury mouse model, p16 deficiency results in an enhanced SMC proliferation underlying intimal hyperplasia.. Moreover, PPAR alpha activation inhibits SMC growth in vivo, and this effect requires p 16 expression. These results identify an unexpected role for p16 in SMC cell-cycle control and demonstrate that PPARa. inhibits SMC proliferation through p16. Thus, the PPAR alpha/p16 pathway may be a potential pharmacological target for the prevention of cardiovascular occlusive complications of atherosclerosis.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
F. Gizard, C. Amant, O. Barbier, S. Bellosta, R. Robillard, F. Percevault, H. Sevestre, P. Krimpenfort, A. Corsini, J. Rochette, C. Glineur, J.C. Fruchart, G. Torpier, B. Staels
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