Calcium Ion Dynamics in Roots: Imaging and Analysis

Calcium sensors are indispensable tools to study the role of Ca2+and visualize Ca2+dynamics during biological processes. Over the past years, the field of Ca2+imaging has strongly expanded by the development of a wide palette of sensors and optimization of sample handling. Here, we provide guidelines for imaging of the Ca2+sensor R-GECO1 in Arabidopsis thaliana roots which can be interpolated to other intensiometric Ca2+sensors. Furthermore, we demonstrate a procedure for image analysis of the acquired time-lapse recordings.