Clinical application of amniotic derived mesenchimal cells conditioned medium in tendon and ligament injuries of sport horses

Growing evidences are indicating that mesenchimal stem cells may promote tendon repair via paracrine-acting molecules targeting inflammatory and trophic processes. To test this hypothesis we have studied the therapeutic potential of conditioned medium secreted by equine amniotic derived cells (AMCs-CM) in sixteen spontaneous different tendon and ligament injuries. Careful monitoring has proved the safety of these implants. Clinical outcomes were favorable and the significantly lower rate (12.5%) of re-injuries observed, compared to horses treated with bone marrow MSC, suggests that treatment with AMCs-CM has a proven efficacy. In conclusion, this study identifies AMCs-CM as a novel therapeutic biological cell-free product in the treatment of horse tendon and ligament diseases.