Nfix Induces a Switch in Sox6 Transcriptional Activity to Regulate MyHC-I Expression in Fetal Muscle
Articolo
Data di Pubblicazione:
2016
Citazione:
Nfix Induces a Switch in Sox6 Transcriptional Activity to Regulate MyHC-I Expression in Fetal Muscle / V. Taglietti, G.A. Maroli, S. Cermenati, S. Monteverde, A. Ferrante, G. Rossi, G. Cossu, M. Beltrame, G. Messina. - In: CELL REPORTS. - ISSN 2211-1247. - 17:9(2016 Nov 22), pp. 2354-2366. [10.1016/j.celrep.2016.10.082]
Abstract:
Sox6 belongs to the Sox gene family and plays a pivotal role in fiber type differentiation, suppressing transcription of slow-fiber-specific genes during fetal development. Here, we show that Sox6 plays opposite roles in MyHC-I regulation, acting as a positive and negative regulator of MyHC-I expression during embryonic and fetal myogenesis, respectively. During embryonic myogenesis, Sox6 positively regulates MyHC-I via transcriptional activation of Mef2C, whereas during fetal myogenesis, Sox6 requires and cooperates with the transcription factor Nfix in repressing MyHC-I expression. Mechanistically, Nfix is necessary for Sox6 binding to the MyHC-I promoter and thus for Sox6 repressive function, revealing a key role for Nfix in driving Sox6 activity. This feature is evolutionarily conserved, since the orthologs Nfixa and Sox6 contribute to repression of the slow-twitch phenotype in zebrafish embryos. These data demonstrate functional cooperation between Sox6 and Nfix in regulating MyHC-I expression during prenatal muscle development.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
MyHC-I; myogenesis; Nfix; Sox6; biochemistry, genetics and molecular biology (all)
Elenco autori:
V. Taglietti, G.A. Maroli, S. Cermenati, S. Monteverde, A. Ferrante, G. Rossi, G. Cossu, M. Beltrame, G. Messina
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