Covalent modifications of albumin Cys34 as a biomarker of mild oxidative dtress

Albumin Cys34 and its oxidized forms -potential biomarkers of oxidative damage; Covalent modifications of albumin Cys34 -as mild oxidative stress biomarker; CYS34 acting -as an antioxidant; Cys34, reactive antioxidant and detoxifying residues -of electrophilic species; Direct infusion MS, infusing solutions of isolated albumin dissolved in denaturing solvent; Human serum albumin (HSA), abundant protein in plasma -60% of total circulating proteins; LC-ESI-MS approach for identification -of unknown and predicted modifications of Cys34; Mercaptoalbumin, markers of antioxidant status; Molecular mechanisms of mercaptoalbumin -as extracellular antioxidant, main oxidation products; Serum albumin concentration and mortality risk-inverse relationship