Dictyostelium Nramp, which is structurally and functionally similar to mammalian DMT1 transporter, mediates phagosomal iron efflux
Articolo
Data di Pubblicazione:
2015
Citazione:
Dictyostelium Nramp, which is structurally and functionally similar to mammalian DMT1 transporter, mediates phagosomal iron efflux / S. Buracco, B. Peracino, R. Cinquetti, E. Signoretto, A. Vollero, F. Imperiali, M. Castagna, E. Bossi, S. Bozzaro. - In: JOURNAL OF CELL SCIENCE. - ISSN 0021-9533. - 128:17(2015 Sep 01), pp. 3304-3316. [10.1242/jcs.173153]
Abstract:
The Nramp (Slc11) protein family is widespread in bacteria and eukaryotes, and mediates transport of divalent metals across cellular membranes. The social amoeba Dictyostelium discoideum has two Nramp proteins. Nramp1, like its mammalian ortholog (SLC11A1), is recruited to phagosomal and macropinosomal membranes, and confers resistance to pathogenic bacteria. Nramp2 is located exclusively in the contractile vacuole membrane and controls, synergistically with Nramp1, iron homeostasis. It has long been debated whether mammalian Nramp1 mediates iron import or export from phagosomes. By selectively loading the iron-chelating fluorochrome calcein in macropinosomes, we show that Dictyostelium Nramp1 mediates iron efflux from macropinosomes in vivo. To gain insight in ion selectivity and the transport mechanism, the proteins were expressed in Xenopus oocytes. Using a novel assay with calcein, and electrophysiological and radiochemical assays, we show that Nramp1, similar to rat DMT1 (also known as SLC11A2), transports Fe(2+) and manganese, not Fe(3+) or copper. Metal ion transport is electrogenic and proton dependent. By contrast, Nramp2 transports only Fe(2+) in a non-electrogenic and proton-independent way. These differences reflect evolutionary divergence of the prototypical Nramp2 protein sequence compared to the archetypical Nramp1 and DMT1 proteins.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Bacterial infection; DMT1; Iron homeostasis; Macropinocytosis; Nramp1; Nramp2
Elenco autori:
S. Buracco, B. Peracino, R. Cinquetti, E. Signoretto, A. Vollero, F. Imperiali, M. Castagna, E. Bossi, S. Bozzaro
Link alla scheda completa:
Link al Full Text: