Association between gene and miRNA expression profiles and stereotyped subset #4 B-cell receptor in chronic lymphocytic leukemia
Articolo
Data di Pubblicazione:
2015
Citazione:
Association between gene and miRNA expression profiles and stereotyped subset #4 B-cell receptor in chronic lymphocytic leukemia / F. Maura, G. Cutrona, L. Mosca, S. Matis, M. Lionetti, S. Fabris, L. Agnelli, M. Colombo, C. Massucco, M. Ferracin, B. Zagatti, D. Reverberi, M. Gentile, A.G. Recchia, S. Bossio, D. Rossi, G. Gaidano, S. Molica, A. Cortelezzi, F. Di Raimondo, M. Negrini, P. Tassone, F. Morabito, M. Ferrarini, A. Neri. - In: LEUKEMIA & LYMPHOMA. - ISSN 1042-8194. - 56:11(2015), pp. 3150-3158.
Abstract:
In this study we investigated specific biological and clinical features associated with chronic lymphocytic leukemia (CLL) patients carrying stereotyped BCR subset #4 (IGHV4-34) among a prospective cohort of 462 CLL/MBL patients in early stage (Binet A). All subset #4 patients (n=16) were characterized by the IGHV mutated gene configuration, and absence of unfavorable cytogenetic lesions, NOTCH1 or SF3B1 mutations. Gene and miRNA expression profiling evidenced that the leukemic cells of subset #4 cases showed significant downregulation of WDFY4, MF2A and upregulation of PDGFA, FGFR1 and TFEC gene transcripts, as well as the upregulation of miR-497 and miR-29c. The transfection of miR-497 mimic in primary leukemic CLL cells induced a downregulation of BCL2, a known validated target of this miRNA. Our data identify biological characteristics associated with subset #4 patients, providing further evidence for the putative role of BCR in shaping the features of the tumor cells in CLL.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
B-cell receptor; BCL2; chronic lymphocytic leukemia; gene expression profiling; microRNA; stereotyped VH CDR
Elenco autori:
F. Maura, G. Cutrona, L. Mosca, S. Matis, M. Lionetti, S. Fabris, L. Agnelli, M. Colombo, C. Massucco, M. Ferracin, B. Zagatti, D. Reverberi, M. Gentile, A.G. Recchia, S. Bossio, D. Rossi, G. Gaidano, S. Molica, A. Cortelezzi, F. Di Raimondo, M. Negrini, P. Tassone, F. Morabito, M. Ferrarini, A. Neri
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