INTRAMAMMARY ADMINISTRATION OF OXYGEN-OZONE MIX IN OIL VEHICLE BLENDED PLATELET CONCENTRATE AS AN UNCONVENTIONAL THERAPY IN BOVINE MASTITIS / FIRST CLINICAL APPLICATION

Biological systems show a high level of complexity. For years single-target drugs have been successful and many pathologies have been considered cured and eradicated. As a matter of fact, the biological complex ecosystem was using the single-target chemical drugs information to evolve and find its own shortcuts. This is the case of antibiotic resistance. Planktonic bacteria evolve generating biofilm communities that are able to gambol antibiotic aggression and possibly evade the host immune system mechanisms. Synergistic solutions are needed to reactivate a virtuous balance in host’s favor: these solutions must be able to cooperate and eventually boost the host immune system, which has a naturally-embedded synergistic modus operandi; they must produce a reduced number of inconvenient metabolites, and they should exert a multifunctional activity. Antimicrobial peptides seem to convey the features required as they deploy a multifunctional antimicrobial set of measures, complying with the host immune system and offering regenerative effects on tissues. On the other end, ozone has been investigated for its bactericidal capacity and for its potential to reactivate depressed immune systems. As this experimental study accounts for, the administration to bovine mastitis of a blending of platelet concentrate, rich in antimicrobial peptides, with oxygen/ozone mix in oil vehicle was examined. Bovine mastitis is a public health issue and its control is fundamental to avoid serious consequences. Prevention should be the first approach to the problem: new predictive protocols and containment means need to be deployed and shared. However feasible effective treatments need to be available. The results of this clinical application point to a promising direction of investigation. In comparison with other therapies based on either a single component or a control group with antibiotic, the blending of platelet concentrate with oxygen/ ozone mix in oil vehicle accomplished the best outcomes in resolving the mastitic occurrence, downgrading and fixing the symptoms, and enhancing animal general conditions. Conceivable explanations for its efficacy have been suggested. Further and deeper research is required.