NF-Y coassociates with FOS at promoters, enhancers, repetitive elements, and inactive chromatin regions, and is stereo-positioned with growth-controlling transcription factors
Articolo
Data di Pubblicazione:
2013
Citazione:
NF-Y coassociates with FOS at promoters, enhancers, repetitive elements, and inactive chromatin regions, and is stereo-positioned with growth-controlling transcription factors / J.D. Fleming, G. Pavesi, P. Benatti, C. Imbriano, R. Mantovani, K. Struhl. - In: GENOME RESEARCH. - ISSN 1088-9051. - 23:8(2013 Aug), pp. 1195-1209.
Abstract:
NF-Y, a trimeric transcription factor (TF) composed of two histone-like subunits (NF-YB and NF-YC) and a sequencespecific subunit (NF-YA), binds to the CCAAT motif, a common promoter element. Genome-wide mapping reveals 5000-15,000 NF-Y binding sites depending on the cell type, with the NF-YA and NF-YB subunits binding asymmetrically with respect to the CCAAT motif. Despite being characterized as a proximal promoter TF, only 25% of NF-Y sites map to promoters. A comparable number of NF-Y sites are located at enhancers, many of which are tissue specific, and nearly half of the NF-Y sites are in select subclasses of HERV LTR repeats. Unlike most TFs, NF-Y can access its target DNA motif in inactive (nonmodified) or polycomb-repressed chromatin domains. Unexpectedly, NF-Y extensively colocalizes with FOS in all genomic contexts, and this often occurs in the absence of JUN and the AP-1 motif. NF-Y also coassociates with a select cluster of growth-controlling and oncogenic TFs, consistent with the abundance of CCAAT motifs in the promoters of genes overexpressed in cancer. Interestingly, NF-Y and several growth-controlling TFs bind in a stereo-specific manner, suggesting a mechanism for cooperative action at promoters and enhancers. Our results indicate that NF-Y is not merely a commonly used proximal promoter TF, but rather performs a more diverse set of biological functions, many of which are likely to involve coassociation with FOS.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Enhancer Elements, Genetic; Promoter Regions, Genetic; Base Sequence; Binding Sites; CCAAT-Binding Factor; Chromatin; Consensus Sequence; Gene Expression Regulation; Gene Ontology; Genome, Human; HeLa Cells; Humans; K562 Cells; Molecular Sequence Annotation; Organ Specificity; Protein Binding; Protein Transport; Proto-Oncogene Proteins c-fos; Terminal Repeat Sequences; Transcription Factors; Transcription Initiation Site
Elenco autori:
J.D. Fleming, G. Pavesi, P. Benatti, C. Imbriano, R. Mantovani, K. Struhl
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