Data di Pubblicazione:
2014
Citazione:
Cigarette Smoke Condensate Affects Monocyte Interaction With Endothelium / I. Giunzioni, A. Bonomo, E. Bishop, S. Castiglioni, A. Corsini, S. Bellosta. - In: ATHEROSCLEROSIS. - ISSN 0021-9150. - 234:2(2014 Jun), pp. 383-390. [10.1016/j.atherosclerosis.2014.03.029]
Abstract:
Objective
Circulating monocytes adhere to the endothelium and migrate into the intima contributing to atherosclerotic plaque growth. Cigarette smoke is a risk factor for atherosclerosis, but it is not completely known how it affects monocyte behavior in atherogenesis.
Methods
We studied the effects of cigarette smoke condensate (CSC) on human monocytes (HM) chemotaxis and transmigration through an endothelial cell (EC) monolayer.
Results
Pre-treatment with CSC caused a decrease in HM chemotaxis and transmigration (-55% and -18% vs control, p<0.05, respectively), paralleled by a reduced expression of Rac 1 GTPase. On the contrary, direct exposure of both HM and EC to CSC increased (+23% vs control, p<0.05) HM transmigration, paralleled by a strong stimulation of VCAM1 and ICAM1 expression by ECs, and by a slight increase in monocyte integrin expression. An enhancement of monocyte transmigration was obtained after the exposure of both HM and EC to medium conditioned by HM previously incubated with CSC (+265% vs control, p<0.001). CSC showed a stimulatory effect on the expression by HM of TLR4, MCP1, IL8, IL1beta, and TNFalfa, which was ablated by pretreatment with PDTC. Incubation with neutralizing antibodies against both MCP1 or IL8 completely abolished the CSC-conditioned medium induced HM transmigration.
Conclusions
CSC induces HM to release chemotactic factor(s), which amplify the recruitment and transmigration of inflammatory cells through EC, but CSC may also reduce HM migratory capacity. Therefore, exposure to CSC affects monocyte behavior and interaction with the endothelium, thus potentially facilitating and/or further aggravating the atherogenic process.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
monocytes ; macrophages ; endothelium ; cigarette smoke ; transmigration
Elenco autori:
I. Giunzioni, A. Bonomo, E. Bishop, S. Castiglioni, A. Corsini, S. Bellosta
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