Preliminary researches to standardize a method of quantitative analysis on Lactobacillus acidophilus in poultry feed

The study focuses on the method and the problems about quantitative analyses in the research on Lactobacillus acidophilus after its addition to commercial poultry-feed, whose rough grinding is not suitable for the "IDF Standard quantitative method for lactic acid bacteria count at 37°" employed in dairy products. Poultry-feed was prepared every month. A sample was collected before and after adding Lactobacillus acidophilus, while analyses were carried out respectively at T 0, 15, and 28 days after the food storage at 4-6°C. The best outcome (more 30% of recovered cells compared to the standard method) resulted from samples subjected to the homogenization and the addition of Skim Milk Powder.