Data di Pubblicazione:
2013
Citazione:
Analytical goals for the determination of HbA₂ / A. Mosca, R. Paleari, B. Wild. - In: CLINICAL CHEMISTRY AND LABORATORY MEDICINE. - ISSN 1434-6621. - 51:5(2013 May), pp. 937-941.
Abstract:
Background: We present a study aimed to define the analytical goals for the determination of hemoglobin A2 , a minor hemoglobin present in human blood normally accounting from 2.5 % to 3.3 % of total hemoglobin, and typically increased up to 6 % - 7 % in subjects carriers of β -thalassemia. Methods: The analytical goals have been derived using two approaches, the first one based on biologic variation, and the second one based on the opinion of experts. Results: The data obtained by studying 17 adult non-carrier healthy subjects, from whom we took blood samples every 2 weeks for 2.0 months, indicated a small intraindividual biologic variation (CVI of 0.7 % ), with respect to a larger between-subject variation (CVG of 7.7 % ). The minimum levels for imprecision, bias and total error derived from the analysis of these data were: 0.5 % , 2.9 % and 4.5 % , respectively. The limits derived from the opinion of experts were based on a questionnaire with three clinical cases, which was circulated among two teams of international experts, and on a discussion about the clinical needs. The average total error derived from such surveys ranged between 7.0 % and 9.5 % . Conclusions: The various methods to derive analytical performance goals gave different limits, thus indicating the need for an increased communication between clinicians and laboratory professionals on this matter.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Analytical goals; Biologic variation; Hemoglobin A2; Reference systems
Elenco autori:
A. Mosca, R. Paleari, B. Wild
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