Errors in ribosomal sequence datasets generated using PCR-coupled 'panbacterial' pyrosequencing, and the establishment of an improved approach
Articolo
Data di Pubblicazione:
2013
Citazione:
Errors in ribosomal sequence datasets generated using PCR-coupled 'panbacterial' pyrosequencing, and the establishment of an improved approach / E.M. Prosdocimi, S. Novati, R. Bruno, C. Bandi, P. Mulatto, R. Giannico, M. Casiraghi, E. Ferri. - In: MOLECULAR AND CELLULAR PROBES. - ISSN 0890-8508. - 27:1(2013 Feb), pp. 65-67. [10.1016/j.mcp.2012.07.003]
Abstract:
Universal bacterial primers are often used in PCR-coupled sequencing approaches to investigate environmental and host-associated bacterial communities. Some of these primers can also amplify eukaryotic DNA. This is leading to the submission of datasets to public databases which are erroneously annotated as prokaryotic sequences. The present note sends a message about the risk of submitting incorrectly annotated sequence data and suggests a reliable approach for the sequencing of 16S rRNA genes and identification of bacteria within complex communities.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
E.M. Prosdocimi, S. Novati, R. Bruno, C. Bandi, P. Mulatto, R. Giannico, M. Casiraghi, E. Ferri
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