Biotechnologica sulphated chondroitin sulphate at position 4 or 6 on the same polysaccharide chain, and process for the preparation thereof

The present invention disclosed a process for the production of chondroitin sulphated with an average molecular weigh (Mw) of 10-30 KDa by chemical sulphation starting from an unsulphated backbone, obtained in turan by acidc hydrolisis of capsular polysaccarides K4 made directly from E. coli strai O5:K4:H14, ordirectly produced from a genetically modified strain of E. coli. Sulphatetion of N-acetil-D-galactosamine residue at position 4 or 6 takes place simultaneously in the same plisaccharide chain, simulating the sulphation pattern observed in natural chondroitin sulphate, unlike the sukphation obtained with the synthesis methods described to date.