Data di Pubblicazione:
2013
Citazione:
Quality of canine spermatozoa retrieved
by percutaneous epididymal sperm
aspiration / S. Varesi, V. Vernocchi, M. Faustini, G.C. Luvoni. - In: JOURNAL OF SMALL ANIMAL PRACTICE. - ISSN 0022-4510. - 54:2(2013 Feb), pp. 87-91. [10.1111/jsap.12020]
Abstract:
OBJECTIVES : To investigate the feasibility of percutaneous epididymal sperm aspiration in dogs and
whether it might provide a population of epididymal spermatozoa similar to the population that can be
obtained by processing isolated epididymis caudae.
METHODS : Concentration and total sperm number, motility, morphology and acrosomal integrity of sper-matozoa retrieved by percutaneous epididymal sperm aspiration, in vitro aspiration and mincing of the
cauda of the epididymis were compared.
RESULTS: Percutaneous epididymal sperm aspiration is a feasible procedure to retrieve a population of
spermatozoa in dogs. Quality is similar to that of spermatozoa collected in vitro, although a wide varia-tion amongst animals was observed.
CLINICAL S IGNIFICANCE : In case of ejaculation failure due to pathological conditions in dogs, the collection
of spermatozoa from the cauda of the epididymis could be an option for providing gametes for assisted
reproductive technologies. Percutaneous epididymal sperm aspiration can be used in dogs with com-promised reproductive performance, in which orchiectomy cannot be performed for medical or owner
reasons. Further studies aimed to investigate whether the percutaneous epididymal sperm aspiration
technique might be feasible for repeated semen collection and to accurately evaluate side effects are
required.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Dog ; spermatozoa ; epididymis ; aspiration ; PESA
Elenco autori:
S. Varesi, V. Vernocchi, M. Faustini, G.C. Luvoni
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