Glia-neuron sandwich cocultures: an in vitro approach to evaluate cell-to-cell communication in neuroinflammation and neurotoxicity
Capitolo
Data di Pubblicazione:
2011
Citazione:
Glia-neuron sandwich cocultures: an in vitro approach to evaluate cell-to-cell communication in neuroinflammation and neurotoxicity / B. Viviani, M. Boraso - In: In Vitro Neurotoxicology / [a cura di] L.G. Costa, G. Giordano, M. Guizzetti. - Clifton : Humana Press, 2011. - ISBN 978-1-61779-170-3. - pp. 135-152
Abstract:
Glia-neuron sandwich cocultures are "in vitro" cell systems suitable to evaluate cell-to-cell interactions relaying on the release of soluble factors, such as proinflammatory cytokines. This chapter presents a protocol to obtain a sandwich coculture from primary rat glial cells and hippocampal neurons. Furthermore, it provides details to evaluate the release of tumor necrosis factor-α by a biological assay and neuronal cell survival, as well as examples of practical application in the investigation of neuroinflammation and its impact on neuronal function.
Tipologia IRIS:
03 - Contributo in volume
Keywords:
Biological assay; MTT-test; Primary glial cells; Primary hippocampal neurons; Sandwich coculture; TNF-α
Elenco autori:
B. Viviani, M. Boraso
Link alla scheda completa:
Titolo del libro:
In Vitro Neurotoxicology