Pectic enzymes from Aureobasidium pullulans LV10

• Aureobasidium pullulans LV 10 produced extracellular pectolytic activity when grown in a medium containing apple pectin as a carbon source. Maximum enzyme production (22 PGU ml−1 and 9 PLU ml−1) was obtained after 4 days of batch growth. Two pectin hydrolases and two pectin lyases by CM-Sepharose 6B, DEAE-cellulose, and Sephadex G-100 column chromatography were partially purified and characterized. Hydrolases I and II gave optimum activity at pH 5.5 and 4.5, respectively, at 50°C. For lyases I and II, the optimum occurred at pH 5.0 and 7.5, respectively, and 40°C.