GM1 ganglioside-Triton X-100 mixed micelles. Transitions among different micellar species monitored by physicochemical and enzymatic methods

Aqueous dispersions of two amphiphiles, GM1 ganglioside and Triton X-100, in different proportions, were analysed for some physicochemical properties (surface tension, viscosity, consolution temperature) and for susceptibility to the action of galactose oxidase. By varying the molar ratio between the two components, well defined transitions among different micellar species were recorded by physicochemical measurements. Galactose oxidase was able to recognize the different species of mixed micelles, its kinetics displayed break points which exactly superimposed on those recorded, under the same conditions, by physicochemical methods.